The Spectrophotometric Determination of Olanzapine via Coupling with Diazotized p-Nitroaniline

  • Sahar R. Fadhel
  • Najwa I. Abdulla
  • Intidhar D. Sulaiman


A new spectrophotometric method has been developed for the assay of olanzapine (OLN.) in pure and dosage forms. The method is based on the diazocoupling of (OLN.) with diazotized p-nitroaniline in alkaline medium to form a stable brown colored water-soluble azo dye with a maximum absorption at 405 nm.  The variables that affect the completion of reaction have been carefully optimized. Beer’s law is obeyed over the concentration range of (0.5-45.0 μg.mL-1) with a molar absorptivity of 1.5777×104  The limit of detection was  0.3148  Î¼g.mL-1 and  Sandell’s  sensitivity value was 0.0198 μ The proposed method has been applied successfully to the determination of olanzapine in tablet pharmaceutical preparations.

Keywords: Spectrophotometry, Olanzapine, Diazotization, p-nitroaniline, tablet dosage form.