Detection of Epicatechin in Camellia sinensis Leaves by Thin Layer Chromatography and High Performance Liquid Chromatography Techniques
DOI:
https://doi.org/10.31351/vol31iss2pp304-312Abstract
The current study performed in order to detect and quantify epicatechin in two tea samples of Camellia sinensis (black and green tea) by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Extraction of epicatechin from black and green tea was done by using two different methods: maceration (cold extraction method) and decoction (hot extraction method) involved using three different solvents which are absolute ethanol, 50% aqueous ethanol and water for both extraction methods using room temperature and direct heat respectively. Crude extracts of two tea samples that obtained from two methods were fractionated by using two solvents with different polarity (chloroform and ethyl acetate). Qualitative and quantitative determinations of epicatechin in tea samples were investigated. Epicatechin identification was made by utilizing preliminary chemical tests and TLC. This identification was also boosted by HPLC and the quantity of epicatechin was determined in all ethyl acetate fractions of two tea samples. This research revealed the existence of epicatechin in black and green tea according to TLC and HPLC. Aqueous ethanol 50% was the best solvent for extraction of epicatechin from leaves of tea. Quantitative estimation of epicatechin by HPLC revealed that ethyl acetate fraction of DGTAE contains the higher concentration of epicatechin than other analyzed fractions. Conclusion, tea is an excellent source of catechins particularly epicatechin that possessed various pharmacological effects.
References
Graham HN. Green tea composition, consumption, and polyphenol chemistry. Preventive Medicine, 1992; 21: 334-350.
Sharangi AB .Medicinal and therapeutic potentialities of tea (Camellia sinensis L.) – A review. Food Research International, 2009; 42:529–535.
Agarwal U, Pathak D P, Bhutani R, Kapoor G, Kant R. Review on Camellia sinensis : Nature’s Gift. International Journal of Pharmacognosy and Phytochemical Research, 2017; 9(8): 1119-1126.
Narotzki B, Reznick AZ, Aizenbud D, Levy Y. Green tea: a promising natural product in oral health. Arch Oral Biol. 2012 May; 57(5):429-35.
Paganini-Costa P and Carvalho-da-Silva D. A cup (tea) of chemistry. Revista Virtual Quimica, 2011; 3: 27-36.
Butt M, Ahmad R, Sultan M, Qayyum M and Naz A. Green Tea and Anticancer Perspectives: Updates from Last Decade. Critical Reviews in Food Science and Nutrition, 2014; 55(6):792-805.
Lambert JD, Elias RJ. The antioxidant and pro-oxidant activities of green tea polyphenols: A role in cancer prevention, Archives of Biochemistry and Biophysics, 2010; 1: 65-72.
Chattopadhyay P, Besra SE, Gomes A, Das M, Sur P, Mitra S, Vedasiromoni JR, Anti-inflammatory activity of tea (Camellia sinensis) root extract, Life sci, 2004; 74(15):1839-1849.
Yen GC, Chen HY, Relationship between antimutagenic activity and major components of various teas. Mutagenesis, 1996; 11(1): 37–41.
Shokrzadeh M, Ebadi AG, Mirshafiee SS, Choudhary MI, Effect of the aqueous green leaf extract of green tea on glucose level of rat, Pakistan journal of biological sciences, 2006; 9(14): 2708- 2711.
Oyejide OO, Olushola L, Hepatoprotective and antioxidant properties of extract of Camellia sinensis (black tea) in rats, African Journal of Biotechnology, 2005; 4(11):1432-1438.
Oliveira RMMD. Quantification of catechins and caffeine from green tea (Camellia sinensis) infusions, extract, and ready-to-drink beverages. Food Science and Technology (Campinas), 2012; 32(1): 163-166.
Row KH, JinY. Recovery of catechin compounds from Korean tea by solvent extraction. Bioresource Technology, 2006; 97: 790–793.
Chang Y, Chamidha Kumarihami H, Kim H and Song K. Current Status and Prospect of Molecular Breeding in Tea Plant (Camellia sinensis). The Korean Tea Society, 2017; 23(1):74-84.
Rashidinejad A, Birch EJ, Hindmarsh J, Everett DW. Molecular interactions between green tea catechins and cheese fat studied by solid-state nuclear magnetic resonance spectroscopy. Food Chem. 2017; 215:228–234.
Raju VSS, Nareshraju N, Kannababu S, and Gottumukkala V. Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography. International Scholarly Research Notices, 2014; 1:1-5.
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