Protective Effect of Cafestol on Doxorubicin-induced Genotoxicity in Rats
DOI:
https://doi.org/10.31351/vol32issSuppl.pp16-25Keywords:
Doxorubicin, Cafestol, Genotoxicity, chromosomal aberration, micronucleus assay, Comet assayAbstract
Doxorubicin is an efficient antineoplastic agent that has a broad antitumour spectrum; however, its genotoxic adverse effects on normal cells can be produced through oxidative damage, and this limits its clinical application. Cafestol is a naturally-occurring diterpene in unfiltered coffee with noteworthy antioxidant, antimutagenic and anti-inflammatory activities.The present study aimed to investigate the possible protective effect of cafestol against doxorubicin-induced chromosomal and DNA damage in rat bone marrow cells. Wistar
Albino rats of both sexes were administered cafestol (5mg/kg body weight once daily for 14 consecutive days) by oral gavage alone or with doxorubicin which was injected as a single dose (90 mg/kg intraperitoneally at day 14) to induce toxicity. The bone marrow was harvested 24 hours after doxorubicin’s injection in all groups for the assessment of structural chromosomal aberration, micronucleus, and comet assays. The result showed that rats in the doxorubicin-only group exhibited a significant decrease (P<0.05) in mitotic index with a significant elevation (P<0.05) in the % DNA in Tail, micronucleus appearance and total structural chromosomal aberrations compared to those of the negative control group; while oral administration of cafestol 14 days prior to doxorubicin, significantly-reduced the % DNA in Tail, micronucleus appearance, and the total number of chromosomal aberrations (P<0.05), and improved the mitotic index compared to rats intraperitoneally-injected with doxorubicin alone.
This study revealed that cafestol has protective effects against the genotoxicity induced by doxorubicin.
References
Young RC, Ozols RF MC. The anthracycline antineoplastic drugs. N Engl J Med. 1981;305:139–53.
Hitchcock-Bryan S, Gelber RD, Cassady JR SS. The impact of induction anthracycline on long-term failure-free survival in childhood acute lymphoblastic leukaemia. Med Pediatr Oncol. 1986;14:211–5.
Vuong JT, Stein-Merlob AF, Cheng RK, Yang EH. Novel Therapeutics for Anthracycline Induced Cardiotoxicity. Frontiers in Cardiovascular Medicine . 2022 ;Vol. 9. Available from: https://www.frontiersin.org/articles/10.3389/fcvm.2022.863314
Shandilya M, Sharma S, Das PP, Charak S. Molecular-Level Understanding of the Anticancer Action Mechanism of Anthracyclines. In: Arnouk H, Hassan BAR, editors. Rijeka: IntechOpen; 2020. p. Ch. 9. Available from: https://doi.org/10.5772/intechopen.94180
Chen, Tzu, Wu, Yan, Chung, Yu, Hwu, Yeukuang, Cheng, Hsun, Mou, Yuan and WL. Probing the Dynamics of Doxorubicin-DNA Intercalation during the Initial Activation of Apoptosis by Fluorescence Lifetime Imaging Microscopy (FLIM). PLoS One. 2012;7:(9): e44947. Available from: https://doi.org/10.1371/journal.pone.0044947
Zhang X-Y, Yang K-L, Li Y, Zhao Y, Jiang K-W, Wang Q, et al. Can Dietary Nutrients Prevent Cancer Chemotherapy-Induced Cardiotoxicity? An Evidence Mapping of Human Studies and Animal Models. Frontiers in Cardiovascular Medicine. 2022; Vol. 9. Available from: https://www.frontiersin.org/articles/10.3389/fcvm.2022.921609
L.M.G. Antunes CST. Effects of high doses of vitamins C and E against doxorubicin-induced chromosomal damage in Wistar rat bone marrow cells. Mutat Res. 1998;419:137–43.
Rodriguez E, Sreekantaiah C CR. Genetic changes in epithelial solid neoplasia. Cancer Res. 1994;54(13):3398–406.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)
This work is licensed under a Creative Commons Attribution 4.0 International License.