Evaluation the Effect of Phytosterol Fraction of Chenopodium Murale in Comparison with Tacrolimus on Mice Induced Atopic Dermatitis
DOI:
https://doi.org/10.31351/vol32iss1pp84-91Keywords:
Phytosterol Fraction,, Interleukin-13, Interleukin-4,, Tacrolimus,, Chenopodium Murale, Atopic dermatitisAbstract
Atopic dermatitis (atopic eczema), is a common familial chronic inflammatory skin disease, determined by xerosis, itching, scaly and erythematous skin lesions, and high serum levels of IgE. Between 10 to 20% of children and 1 to 3% of adults worldwide affected by it and has negative medical and social effect on patients and their families. To evaluate the effectiveness of Phytosterol Fraction of Chenopodium Murale on induced atopic dermatitis (AD) of mice; Forty mice were included in the study, divided in to four groups (10 mice/group): apparently healthy, induced AD without treatment, induced AD treated with Tacrolimus 0.1% ointment, and induced AD treated with Phytosterol Fraction of Chenopodium Murale cream 3% topically. Examination of histopathology was done and skin homogenates levels also measured using Mann Whitney U test to determine meanSD. Levels of WBC, Eosinophil, skin tissue homogenate of IL-13 and IL-4, serum IgE, and histopathological scores were significantly increased among induced non treated AD group in comparison with control group. Comparisons of non-treated induced AD group with Chenopodium Murale or Tacrolimus treated groups; shows a significant reduction in the levels of all studied parameters’ (WBC, Eosinophil, skin tissue homogenate of IL4- and IL-13, serum IgE, observational severity score, and histopathological scores) after the application of Tacrolimus 0.1% ointment or Chenopodium Murale cream 3% topically. The comparison between the effect of topical application of tacrolimus and Phytosterol Fraction on the studied variables shows that the level of WBC and thickness of epidermis and inflammatory cells were significantly lower after tacrolimus treatment, while high significant reduction was founded in parakeratosis and score of observational severity among Chenopodium murale treated group in comparison with Tacrolimus treated group. In conclusion, the use of these therapeutic agents that target IgE, IL-4 and IL-13 could be promising in the treatment of AD.
References
Fuxench ZCC. Atopic dermatitis: disease background and risk factors. In Management of Atopic Dermatitis. Springer, Cham, 2017;1027:11-19.
Spergel JM. Epidemiology of atopic dermatitis and atopic march in children. Immunol Allergy Clin North Am. 2010;30(3):269-280.
Nutten S. Atopic Dermatitis: Global Epidemiology and Risk Factors. Ann Nutr Meta, 2015; 66(1):8-16.
Tanei, R. Atopic Dermatitis in Older Adults: A Review of Treatment Options. Drugs Aging 2020:37(3):149-160.
Astellas Pharma US. Patient information sheet: Protopic 0.1% (tacrolimus), NDC 2006; 0469-5202-30.
Lee JH, Son SW, Cho SH. A comprehensive review of the treatment of atopic eczema. Allergy Asthma Immunol Res, 2016; 8(3):181–190.
Nghiem P, Pearson G, Langley RG: Tacrolimus and pimecrolimus: from clever prokaryotes to inhibiting calcineurin and treating atopic dermatitis. J Am Acad Dermatol, 2002; 46 (2):228-241.
Kang S, Lucky AW, Pariser D, Lawrence I, Hanifin JM. Long-term safety and efficacy of tacrolimus ointment for the treatment of atopic dermatitis in children. J Am Acad Dermatol , 2001;44(1):58–64
Singh KP, Dwevedi AK, Dhakre G, Evaluation of antibacterial activities of Chenopodium album. IJABPT, 2011;2(3):398-401.
Ahmed A A, Abu-Raghif A R. Effect of Topical Phytosterol Fraction of Chenopodium murale on Induced Hypertrophic Scar in Rabbits. Journal of Global Pharma Technology, 2020;12(02):115-124
Aldini R, Micucci M, Cevenini M, Fato R, Bergamini C, Nanni C, et al. Antiinflammatory effect of phytosterols in experimental murine colitis model: prevention, induction, remission study. PloS one, 2014;9(9), e108112.
Ahmad B, Jan Q, Bashir S, Nisar M, Shaheen F, Ahmad M. Pharmacological and biological investigations of Chenopodium murale Linn. Asian Journal of Plant Sciences, 2003;2(15-16), pp.1107-1111.
Batcha O, Gnatoulma K, Gérard T, Laura L, Efui G, Manuel R, et al. Anti-inflammatory, antibacterial and antioxidant activities of Chenopodium ambrosioides L. (Chenopodiaceae) extracts 2021;162: 16764 - 16794 .10.35759/JABs.162.7.ISSN 1997-5902.
Kim H, Kim JR, Kang H, Choi J, Yang H, Lee P, et al. 7,8,49-Trihydroxyisoflavone Attenuates DNCB-Induced Atopic Dermatitis-Like Symptoms in NC/Nga Mice. PLoS ONE 2014;9(8):e104938.
Harborne J.B. Phytochemical Methods, A Guide to Modern Techniques of Plant Analysis.1st ed. London: Chapman and Hall, New York, 1979;278.
Mohammed NJ, Wisam A. Ameen W A. 2015. The effect of topical finasteride in treatment of idiopathic hirsutism. AJBM 2015; 3(9):552 – 566
Han JS, Won KH, Chang SE, Kim JE. Tacrolimus 0.1% ointment in the treatment of allergic contact dermatitis: a new approach. Int J Dermatol, 2014;53: e470-e471.
TrivellatoGrassi L, Malheiros A, Meyre-Silva C, Buss Z, Monguilhott E D, Fröde T S, et al. From popular use to pharmacological validation: A study of the anti-inflammatory, anti-nociceptive and healing effects of Chenopodium ambrosioides extract, Journal of Ethnopharmacology 2013;145(1):127-138.
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