The Hepatoprotective Effect of Omega-7 Against Paracetamol-Induced Hepatotoxicity in Male Rats
DOI:
https://doi.org/10.31351/vol32iss3pp85-94Keywords:
Key words: Hepatotoxicity, Omega-7, Oxidative stress, ParacetamolAbstract
Abstract
Paracetamol toxicity, whether accidental or not is a worldwide issue that leads to hepatotoxicity, acute liver failure, as well as irreversible liver injury requiring liver transplantation. Omega-7 is a monounsaturated fatty acid with a number of beneficial properties. The aim of the present study was to assess the potential protective role of omega-7 fatty acid against hepatotoxicity induced by paracetamol in male rats. Thirty male Rats were separated into five groups (six rats in each group) and received the following treatment: group 1 received liquid paraffin orally via gavage tube for seven days successively, group 2 received liquid paraffin orally via gavage tube for seven days successively, and on day eight, rats received a single intraperitoneal injection of paracetamol (500 mg/kg/day), group 3 received omega-7 (300 mg/kg/day) orally via gavage tube for seven days successively, group 4 received omega-7 (100 mg/kg/day) orally via gavage tube for seven days successively, and on day eight, rats received a single intraperitoneal injection of paracetamol (500 mg/kg/day), group 5 received omega-7 (300 mg/kg/day) orally via gavage tube for seven days successively, and on day eight, rats received a single intraperitoneal injection of paracetamol (500 mg/kg/day). On day nine, rats have been sacrificed by cervical dislocation and liver homogenate samples were collected for analysis. This study showed that there was a significant decrease in hepatic superoxide dismutase, catalase, glutathione-peroxidase and glutathione levels, accompanied with significant increase in hepatic malondialdehyde level in paracetamol group compared to the negative control group. However, administration of omega-7 resulted in significant increase in hepatic superoxide dismutase, catalase and glutathione levels and significant decrease in hepatic malondialdehyde level compared to paracetamol group. In conclusion, omega-7 has a protective effect on paracetamol-induced hepatotoxicity in rats.
Keywords: Antioxidant, Hepatotoxicity, Omega-7, Oxidative stress, Paracetamol.
References
AlZubaidi RF, Al-Shawi NN. The Impact of Two Doses of Vitamin K2 (Menaquinone-7) on Doxorubicin-Induced Hepatotoxicity in Rats. Iraqi Journal of Pharmaceutical Sciences. 2017; 25:22-8.
Parameswari SA, Chetty CM, Chandrasekhar KB. Hepatoprotective activity of Ficus religiosa leaves against isoniazid+ rifampicin and paracetamol induced hepatotoxicity. Pharmacognosy Research. 2013;5(4):271.
Yan M, Huo Y, Yin S, Hu H. Mechanisms of acetaminophen-induced liver injury and its implications for therapeutic interventions. Redox Biol. 2018; 17:274–83.
Simões C, Santos S, Vicente M, Sousa Cardoso F. Epidemiology of Acute Liver Failure from a Regional Liver Transplant Center in Portugal. GE Port J Gastroenterol. 2018 ;26(1):33–39.
Azarmehr N, Afshar P, Moradi M, Sadeghi H, Sadeghi H, Alipoor B, et al. Hepatoprotective and antioxidant activity of watercress extract on acetaminophen-induced hepatotoxicity in rats. Heliyon. 2019;5(7):02072.
Li S, Hong M, Tan HY, Wang N, Feng Y. Insights into the role and interdependence of oxidative stress and inflammation in liver diseases. Oxidative medicine and cellular longevity. 2016; 2016:21.
Wang X, Wu Q, Liu A, Anadón A, Rodríguez J-LL, Martínez-Larrañaga M-RR, et al. Paracetamol: overdose-induced oxidative stress toxicity, metabolism, and protective effects of various compounds in vivo and in vitro. Drug Metab Rev. 2017;49(4):395–437.
Bouhlali ED, Derouich M, Hmidani A, Bourkhis B, Khouya T, Filali-Zegzouti Y, Alem C. Protective effect of Phoenix dactylifera L. seeds against paracetamol-induced hepatotoxicity in rats: a comparison with vitamin C. The Scientific World Journal. 2021; 2021:6618273.
Rotundo L, Pyrsopoulos N. Liver injury induced by paracetamol and challenges associated with intentional and unintentional use. World journal of hepatology. 2020 ;12(4):125.
Carvalho NR, Da Rosa EF, Da Silva MH, Tassi CC, Corte CLD, Carbajo-Pescador S, et al. New therapeutic approach: Diphenyl diselenide reduces mitochondrial dysfunction in acetaminophen-induced acute liver failure. PLoS One. 2013;8(12):81961.
Kumari A, Kakkar P. Lupeol prevents acetaminophen-induced in vivo hepatotoxicity by altering the Bax/Bcl-2 and oxidative stress-mediated mitochondrial signaling cascade. Life sciences. 2012 ;90(15-16):561-570.
Walker CG, Browning LM, Stecher L, West AL, Madden J, Jebb SA, Calder PC. Fatty acid profile of plasma NEFA does not reflect adipose tissue fatty acid profile. British Journal of Nutrition. 2015;114(5):756-762.
Walker CG, West AL, Browning LM, Madden J, Gambell JM, Jebb SA, Calder PC. The pattern of fatty acids displaced by EPA and DHA following 12 months supplementation varies between blood cell and plasma fractions. Nutrients. 2015 ;7(8):6281-6293.
de Souza CO, Vannice GK, Rosa Neto JC, Calder PC. Is Palmitoleic Acid a Plausible Nonpharmacological Strategy to Prevent or Control Chronic Metabolic and Inflammatory Disorders? Mol Nutr Food Res. 2018;62(1): 1700504.
Bolsoni-Lopes A, Festuccia WT, Chimin P, Farias TS, Torres-Leal FL, Cruz MM, et al. Palmitoleic acid (n-7) increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation. Lipids Health Dis. 2014;13(1):1-10.
Yang ZH, Miyahara H, Hatanaka A. Chronic administration of palmitoleic acid reduces insulin resistance and hepatic lipid accumulation in KK-Ay Mice with genetic type 2 diabetes. Lipids in Health and disease. 2011 Dec;10(1):1-8.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)
This work is licensed under a Creative Commons Attribution 4.0 International License.
