Isolation, Identification, and Quantification of Two Compounds from Cassia glauca Cultivated in Iraq

Authors

  • Shamam Kanaan M Abdlkareem Ministry of Health and Environment, Health Directorate of Al_Karkh, Baghdad, Iraq.
  • Enas Jawad Kadhim kadhim Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad

DOI:

https://doi.org/10.31351/vol32iss3pp95-104

Keywords:

Cassia glauca, luteolin, chlorogenic acid, HPLC, and LC-MS/MS Q TOF.

Abstract

The Cassia glauca Lam. is the tree that belongs to the Fabaceae family and is native to India has many uses in indigenous systems of medicine, folk medicine, and traditional Brazilian medicine. Has many pharmacological activities such as anti-diabetic, antibacterial, antifungal, antioxidant, anti-hemolytic, anticancer, cardio-protective, and Hepato-protection.  The aim of study is to Isolation, identification, and quantification of some compounds from aerial parts of Cassia glauca since no phytochemical investigation had previously been done in Iraq for this plant. The aerial parts were defatted in n. hexane for 48 hours. The defatted materials were extracted in 85% ethanol using the hot method (soxhlet), then the extract was fractionated using different solvents (chloroform, ethyl acetate, and n-butanol). High-performance liquid chromatography (HPLC), was used for identification and quantification by using authenticated standards, and preparative layer chromatography (PLC) was used for the isolation of the identified compounds. The isolated compounds were identified after isolation by liquid chromatography Mass Spectrometry LC-MS/MS-Q-TOF method. The different chromatographic and spectroscopic methods results indicate the presence of luteolin and chlorogenic acid in the ethyl acetate fraction and n-butanol fraction respectively and estimated the quantity as (130.77µg/1g) for luteolin, and (0.0006%) for chlorogenic acid from 50g of plant material.

References

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Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi, India: Published by NAG publishers; 2002. p. 54.

Singh, R.B. Nature of seed polysaccharide isolated from Cassia glauca Lam. plant. Nature. SERBD-International Journal of Multidisciplinary Sciences, 2018 1-2.

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Published

2023-12-30

How to Cite

1.
Kanaan M Abdlkareem S, kadhim EJK. Isolation, Identification, and Quantification of Two Compounds from Cassia glauca Cultivated in Iraq. Iraqi Journal of Pharmaceutical Sciences [Internet]. 2023 Dec. 30 [cited 2024 Nov. 5];32(3):95-104. Available from: https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/2117