Study The Effects of 5 Selenocyantouracil Compound on The liver and Kidney of Male Rats After Acetaminophen-Induced Liver Injury


  • Sahar Muayad Noori Department Pharmacology and Toxicology, College of Pharmacy, University of Basrah, Basrah, Iraq.
  • Nadheerah Falih Neamah Department Pharmacology and Toxicology, College of Pharmacy, University of Basrah, Basrah, Iraq.
  • Shaker Abdalsalim Al-Jadaan Department Pharmaceutical Chemistry, College of Pharmacy, University of Basrah, Basrah, Iraq.



5-selenacyanatouracil, Acetaminophen, ALT, AST, Creatinine, Urea, Histopathology


Selenium compounds have long been assumed to protect organs against inflammation and cancer development by reducing oxidative stress. Drug-induced liver injury is a common side effect of many medications, with main symptoms ranging from an increase in asymptomatic liver enzymes to liver failure such as acetaminophen (AAP). The main objective of the present work is the protective effect of 5 selenocyantouracil (5SeU) against liver and kidney damage induced by AAP. Fifty male rats were divided into 4 groups consisting:  Group A: rats were given only food and distal water (DW). Group B: rats were treated with acetaminophen (500mg/kg). Group C: rats were given acetaminophen in the preceding dose, and 5SeU (50 mg/kg). Group D: rats were treated with acetaminophen orally at the prior dose, and 5SeU (100mg/kg) orally once daily for 21 days. The results revealed significant differences of liver enzymes, urea and creatinine values at 5SeU treated groups in comparison with AAP group.  Histopathological examinations confirm such results. As a conclusion, 5SeU compound has a protective activity against AAP-induced tissue damage.


Series C. In Vitro and in Vivo Measurements of ROS Scavenging Activity and White Blood Cells Activity by Chemiluminescence of a New Selena- Diazole Derivative Compare to Dipyrone Activity In Vitro and in Vivo Measurements of ROS Scavenging Activity and White Blood . 2021;

Minich WB. Selenium Metabolism and Biosynthesis of Selenoproteins in the Human Body. Biochemistry (Moscow). 2022;87:S168–77.

Hosny NS, Hashem NM, Morsy AS, Abo-elezz ZR. Effects of Organic Selenium on the Physiological Response, Blood Metabolites, Redox Status, Semen Quality, and Fertility of Rabbit Bucks Kept Under Natural Heat Stress Conditions. Frontiers in Veterinary Science. 2020;7(June):1–14.

Regina BF, Gladyshev VN, Arnér ES, Berry MJ, Bruford EA, Burk RF, et al. Selenoprotein gene nomenclature. Journal of Biological Chemistry. 2016;291(46):24036–40.

Huang X, Dong YL, Li T, Xiong W, Zhang X, Wang PJ, et al. Dietary selenium regulates micrornas in metabolic disease: Recent progress. Nutrients. 2021;13(5):1–14.

Gorini F, Sabatino L, Pingitore A, Vassalle C. Selenium: An element of life essential for thyroid function. Molecules. 2021;26(23):1–14.

MUHAMMAD‑ALI MA, HAMZA SALMAN H, JASIM E. Antioxidant Activity of Some Newly Prepared Symmetrically Azo Dyes Derived From Sulfa Drugs. Asian Journal of Pharmaceutical and Clinical Research. 2019;12(2):479–83.

Liu Q, Du P, Zhu Y, Zhang X, Cai J, Zhang Z. Thioredoxin reductase 3 suppression promotes colitis and carcinogenesis via activating pyroptosis and necrosis. Cellular and Molecular Life Sciences. 2022;79(2).

Neamah NF, Naaeem Khudair AR, Al-Jadaan SAN. Comparative evaluation of biochemical profiling of 4’, 4"- (4, 5, 6, 7-TETRAHYDRO- [1, 2, 3-] SELENADIAZOLO [4, 5e] PYRIDINE-4, 6-DIYL) BIS (BENZENE-1, 3-DIOL) and dipyrone on female rat’s. Journal of Physics: Conference Series. 2019;1279(1).

Elsayed Azab A, A Adwas Almokhtar, Ibrahim Elsayed AS, A Adwas A, Ibrahim Elsayed Ata Sedik, Quwaydir FA. Oxidative stress and antioxidant mechanisms in human body. Journal of Applied Biotechnology & Bioengineering. 2019;6(1):43–7.

AL-Aloosy YAM, AL-Tameemi AJH, Jumaa SS. The role of enzymatic and non-enzymatic antioxidants in facing the environmental stresses on plant : A review. Plant Archives. 2019;19(June):1057–60.

MAYSAA BANAY ZUBAIRI. Metoprolol-Induced Liver Injury and the Hepatoprotective Role of Vitamin E in Rabbits. Asian Journal of Pharmaceutical and Clinical Research. 2019;12(4):145–8.

Jaccob AA, Ahmed ZH, Aljasani BM. Vitamin C, omega-3 and paracetamol pharmacokinetic interactions using saliva specimens as determiners. 2019;30(5). Available from:

Wang X, Wu Q, Liu A, Anadón A, Rodríguez J-L, Martínez-Larrañaga M-R, et al. Paracetamol: overdose-induced oxidative stress toxicity, metabolism, and protective effects of various compounds in vivo and in vitro. Drug metabolism reviews. 2017 Nov;49(4):395–437.

McGill MR. The past and present of serum aminotransferases and the future of liver injury biomarkers. EXCLI Journal [Internet]. 2016;15:817–28. Available from:

Chen SL, Li JP, Li LF, Zeng T, He X. Elevated preoperative serum alanine aminotransferase/aspartate aminotransferase (ALT/AST) ratio is associated with better prognosis in patients undergoing curative treatment for gastric adenocarcinoma. International Journal of Molecular Sciences. 2016;17(6):1–11.

Yokoyama M, Watanabe T, Otaki Y, Takahashi H, Arimoto T, Shishido T, et al. Association of the aspartate aminotransferase to alanine aminotransferase ratio with BNP level and cardiovascular mortality in the general population: The Yamagata study 10-year follow-up. Disease Markers. 2016;2016.

Wang HL, Chu CH, Tsai SJ, Yang RJ. Aspartate aminotransferase and alanine aminotransferase detection on paper-based analytical devices with inkjet printer-sprayed reagents. Micromachines. 2016;7(1):1–10.

Kasarala G, Tillmann HL. Standard liver tests. Clinical Liver Disease. 2016;8(1):13–8.

Bamanikar S, Bamanikar A, Arora A. Study of Serum urea and Creatinine in Diabetic and non-diabetic patients in in a tertiary teaching hospital. The Journal of Medical Research. 2016;2(1):12–5.

Lalla P, Banu Pathan S, Jawade P. Correlation of Serum Urea and Serum Creatinine in Diabetics patients and normal individuals. International Journal of Clinical Biochemistry and Research. 2020;7(1):45–8.

Higgins C. Urea and the clinical value of measuring blood urea concentration. Radiometer Medical ApS [Internet]. 2016;(August):1–6. Available from:

Sahi DN. A Comparative Study of Serum C-Reactive Protein, Urea and Creatinine Levels in Chronic Kidney Disease Patients with Normal Subjects. journal of Medical Science And clinical Research. 2019;7(3):1480–3.

Majeed SK, Ramadhan MA, Monther W. Long-term toxicological effects of paracetamol in rats. Iraqi Journal of Veterinary Sciences. 2013;27(1):65–70.

Hasan KMM, Tamanna N, Haque MA. Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed. Food Science and Human Wellness [Internet]. 2018;7(1):77–82. Available from:

Alahmadi BA, El-Alfy SH, Hemaid AM, Abdel-Nabi IM. The protective effects of vitamin E against selenium-induced oxidative damage and hepatotoxicity in rats. Journal of Taibah University for Science [Internet]. 2020;14(1):709–20. Available from:

Łanocha-Arendarczyk N, Baranowska-Bosiacka I, Kot K, Pilarczyk B, Tomza-Marciniak A, Kabat-Koperska J, et al. Biochemical profile, liver and kidney selenium (Se) status during acanthamoebiasis in a mouse model. Folia Biologica (Poland). 2018;66(1):33–40.

Reja M, Makar M, Visaria A, Marino D, Rustgi V. Increased serum selenium levels are associated with reduced risk of advanced liver fibrosis and all-cause mortality in NAFLD patients: National Health and Nutrition Examination Survey (NHANES) III. Annals of Hepatology. 2020;19(6):635–40.

Ozardali I, Bitiren M, Karakilçik AZ, Zerin M, Aksoy N, Musa D. Effects of selenium on histopathological and enzymatic changes in experimental liver injury of rats. Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 2004 Oct;56(1–2):59–64.

Zwolak I. The Role of Selenium in Arsenic and Cadmium Toxicity: an Updated Review of Scientific Literature. Biological Trace Element Research. 2020;193(1):44–63.

Gounden V, Bhatt H, Jialal I. Renal Function Tests [Internet]. University of KwaZulu Natal: StatPearls Publishing, Treasure Island (FL); 2021. Available from:

Reshi MS, Yadav D, Uthra C, Shrivastava S, Shukla S. Acetaminophen-induced renal toxicity: preventive effect of silver nanoparticles. Toxicology Research. 2020;9(4):406–12.

Refaie AAER, Ramadan A, Mossa ATH. Oxidative damage and nephrotoxicity induced by prallethrin in rat and the protective effect of Origanum majorana essential oil. Asian Pacific Journal of Tropical Medicine. 2014;7(S1):S506–13.

Omrani H, Golmohamadi S, Pasdar Y, Jasemi K, Almasi A. Effect of selenium supplementation on lipid profile in hemodialysis patients. Journal of Renal Injury Prevention. 2016;5(4):179–82.

El-Shenawy SMA, Hassan NS. Comparative evaluation of the protective effect of selenium and garlic against liver and kidney damage induced by mercury chloride in the rats. Pharmacological Reports. 2008;60(2):199–208.

Musik I, Kiełczykowska M, Donica H. The influence of selenium compounds of different structure on morphology, blood biochemistry and phagocytic capability of granulocytes in rats. Roczniki Państwowego Zakładu Higieny. 2013;64(2):117–22.

Hasanvand A, Abbaszadeh A, Darabi S, Nazari A, Gholami M, Kharazmkia A. Evaluation of selenium on kidney function following ischemic injury in rats; protective effects and antioxidant activity. Journal of Renal Injury Prevention [Internet]. 2017;6(2):93–8. Available from:

Krishnan V, Loganathan C, Thayumanavan P. Green synthesized selenium nanoparticles using Spermacoce hispida as carrier of s-allyl glutathione: to accomplish hepatoprotective and nephroprotective activity against acetaminophen toxicity. Artificial Cells, Nanomedicine and Biotechnology [Internet]. 2019;47(1):56–63. Available from:

Muhammad-Azam F, Nur-Fazila SH, Ain-Fatin R, Noordin MM, Yimer N. Histopathological changes of acetaminophen-induced liver injury and subsequent liver regeneration in BALB/C and ICR mice. Veterinary World. 2019;12(11):1682–8.

Canayakin D, Bayir Y, Kilic Baygutalp N, Sezen Karaoglan E, Atmaca HT, Kocak Ozgeris FB, et al. Paracetamol-induced nephrotoxicity and oxidative stress in rats: the protective role of Nigella sativa. Pharmaceutical Biology. 2016;54(10):2082–91.

Neamah NF, Al-jadaan SAN. Diol ) on female rats in comparison with Dipyrone . 2019;(April).

Ansar S, Alshehri SM, Abudawood M, Hamed SS, Ahamad T. Antioxidant and hepatoprotective role of selenium against silver nanoparticles. International Journal of Nanomedicine. 2017;12:7789–97.

Data C. Association between Selenium Status and Chronic Kidney Disease in Middle-Aged and Older Chinese Based on. 2022;

Neamah NF, Al-Jadaan SAN, Al-Ali AM. Study of some of novel selena-diazole derivative activities on hematological parameters, differentiate lymphocytes cells in addition to thyroid hormones levels in female rats (One of the series of studies on the impact of the new compound). Systematic Reviews in Pharmacy. 2020;11(11):975–80.

Hiragi S, Yamada H, Tsukamoto T, Yoshida K, Kondo N, Matsubara T, et al. Acetaminophen administration and the risk of acute kidney injury: A self-controlled case series study. Clinical Epidemiology. 2018;10:265–76.

Tsekhmistrenko SI, Bityutskyy VS, Tsekhmistrenko OS, Kharchishin VM, Tymoshok NO, Demchenko AA, et al. nanocompounds. 2021;11(3):199–204.