Synthesis, Characterization and Anticancer Activity of Chitosan Schiff Base / PEG Blend Doped with Gold and Silver Nanoparticles in Treatment of Breast Cancer Cell Line MCF-7

Authors

  • Maha Aldabbagh College of Education for pure science/ Ibn Alhaitham- University of Baghdad
  • Baker F. Abdallaha Department of Chemistry, College of Science, University of Anbar, Ramadi, Iraq.
  • Nibras Y. Abdulla Department of Chemistry, Education College for women, University of Anbar, Ramadi, Iraq
  • Ibraheem J. Ibraheem Department of Chemistry, College of Science, University of Anbar, Ramadi, Iraq.

DOI:

https://doi.org/10.31351/vol33iss2pp101-111

Keywords:

Anticancer cell line, Chitosan, Chitosan Schiff base, PEG, Nano composite

Abstract

   In the present study, chitosan Schiff base has been prepared from chitosan reaction with the para chloro benzaldehyde. The AuNPs and AgNPs were manufacture by extract of onion peels as a reducing agent. The AuNPs and AgNPs that have been synthesized were characterized through UV-vis spectroscopy the XRD analyses and SEM microscopy. The polymer blends of the chitosan / PEG has been prepared through using the approach of solution casting. Chitosan Schiff base / PEG Au and Ag nano-composites were synthesized nano composites and polymer blends have been characterized by FTIR which confirm the formation of Schiff base by revealing a new band of absorption at 1693 cm-1 as a result of the (C=N) imine group. FESEM, DSC and TGA confirms the thermal stability of the prepared polymer blends and nanocomposites, nano composites have shown good results in inhibiting breast cancer cell line MCF-7, IC50 of nanocomposite = 19.44 µg / mL.

Received 10/4/2023

Accepted 26/6/2023

Published 27/6/2024

References

George, A.; Sanjay, M.R.; Srisuk, R.; Parameswaranpillai, J.; Siengchin, A. "A comprehensive review on chemical properties and applications of biopolymers and their composites". Int. J. Biol. Macromol. 2020; 154: 329–338.

Varun, T. K., Senani, S., Jayapal, N., Chikkerur, J., Roy, S., Tekulapally, V. B., Kumar, N. "Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect". Veterinary world.2017; 10(2): 170.

Almukhtar, J. G. J., & Karam, F. F. "Preparation characterization and application of Chitosan nanoparticles as drug carrier". In Journal of Physics: Conference Series.2020; (1664) 1: 012071.

Vhanale, B. T., Deshmukh, N. J., & Shinde, A. T. "Synthesis, characterization, spectroscopic studies and biological evaluation of Schiff bases derived from 1–hydroxy-2-acetonapthanone". Heliyon. 2019; 5(11): e02774. ‏

Ajitha, A. R., & Thomas, S. "Introduction: Polymer blends, thermodynamics, miscibility, phase separation, and compatibilization". In Compatibilization of polymer blends.2020; 1-29.

Li, Z., Shan, P., Li, D., & Zou, X. "Synthesis of branched poly (ethylene glycol) by an acetal protection method". Polymer Science, Series B.2019; 61: 294-301.

Sharma, P. A. W. A. N., & Bhargava, M. A. N. I. S. H. "Applications and characteristics of nanomaterials in industrial environment". Res Dev (IJCSEIERD).2013; 3(4): 63-72.

Slepička, P., Slepičková Kasálková, N., Siegel, J., Kolská, Z., & Švorčík, V. "Methods of gold and silver nanoparticles preparation". Materials.2019;13(1): 1.

Gherasim, O., Puiu, R. A., Bîrcă, A. C., Burdușel, A. C., & Grumezescu, A. M. "An updated review on silver nanoparticles in biomedicine". Nanomaterials.2020; 10(11): 2318.

Pandey, N., Shukla, S. K., & Singh, N. B. "Water purification by polymer nanocomposites: an overview". Nanocomposites. 2017; 3(2): 47-66.

Dai, X., Cheng, H., Bai, Z., & Li, J. "Breast cancer cell line classification and its relevance with breast tumor subtyping". Journal of Cancer. 2017; 8(16): 3131.

Damiri F, Bachra Y, Bounacir C, Laaraibi A, Berrada M. "Synthesis and characterization of lyophilized chitosan-based hydrogels cross-linked with benzaldehyde for controlled drug release". J Chem. 2020; Article ID 8747639.

Iqbal DN, Tariq M, Khan SM, Gull N, Iqbal SS, Aziz A, Iqbal M. "Synthesis and characterization of chitosan and guar gum based ternary blends with polyvinyl alcohol". Int. J. boil. macromol. 2020; 143: 546-554.

Santhosh A, Theertha V, Prakash P, Chandran SS. "From waste to a value added product: Green synthesis of silver nanoparticles from onion peels together with its diverse applications". Mater. Today: Proc. 2020; ‏46:4460-4463.

El-Borady, O. M., Ayat, M. S., Shabrawy, M. A., & Millet, P. "Green synthesis of gold nanoparticles using Parsley leaves extract and their applications as an alternative catalytic, antioxidant, anticancer, and antibacterial agents". Advanced Powder Technology. 2020; 31(10): 4390-4400. ‏

Hassan, O. M., Ibraheem, I. J., Adil, B. H., Obaid, A. S., & Salih, T. A. "Synthesis of Silver Nanoparticles by ecofriendly nvironmental method using Piper nigrum, Ziziphus spina-christi, and Eucalyptusglobulus extract". In Journal of Physics: Conference Series. 2020; (1530), 1: 012139.

Hezma, A. M., Rajeh, A., & Mannaa, M. A. "An insight into the effect of zinc oxide nanoparticles on the structural, thermal, mechanical properties and antimicrobial activity of Cs / PVA composite". Colloids and Surfaces A: Physicochemical and Engineering Aspects, 581,. 2019; 123821.

Al-Ziaydi AG, Al-Shammari AM, Hamzah MI, Jabir MS. "Hexokinase inhibition using D-Mannoheptulose enhances oncolytic newcastle disease virus-mediated killing of breast cancer cells". Cancer Cell Int. 2020; 20(1):1-10.

Al-Ziaydi AG, Hamzah MI, Al-Shammari AM, Kadhim HS, Jabir MS. "The anti-proliferative activity of D-mannoheptulose against breast cancer cell line through glycolysis inhibition". AIP Conference Proceedings. 2020; 2307 (1) :020023.

Al Salman HN K, Ali ET, Jabir M, Sulaiman GM, Al Jadaan SA. "Benzhydrylsulfinyl N hydroxyacetamide Na extracted from fig as a novel cytotoxic and apoptosis inducer in SKOV 3 and AMJ 13 cell lines via P53 and caspase 8 pathway".‏ Eur food Res Technol. 2020; 246:1591–1608.

Al-Ziaydi AG, Al-Shammari AM, Hamzah MI, Kadhim HS, Jabir MS. "Newcastle disease virus suppress glycolysis pathway and induce breast cancer cells death". VirusDisease. 2020; 31(3):341-348.

Kareem SH, Naji AM, Taqi ZI, Jabir MS. "Poiyvinylpyrrolidone Loaded MnZnFe2O4 Magnetic Nanocomosites Induce Apoptosis in Cancer Cells Through Mitochondrial Damage and P53 Pathway". J Inorg Organ0met Polym. 2020; 30: 5009-5023.

Jabir MS, Hussien AA, Sulaiman GM, Yaseen NY, Dewir YH, Alwahibi MS, Rizwana H. "Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction". Artif Cells Nanomed Biotechnol. 2021; 49(1): 48-60.

Al-Shammari AM, Al-Saadi H, Al-Shammari SM, Jabir MS. "Galangin enhances gold nanoparticles as anti-tumor agents against ovarian cancer cells". In AIP Conference Proceedings. 2020; 2213 (1) :020206.

Majid SJ, Yasmin MS, Ghassan MS, Nahi YY, Usama IS, Yaser HD. "Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via Enhanced Autophagy". Nanomaterials. 2021; 11: 384. 1-22.

Khashan KS, Abdulameer FA, Jabir MS, Hadi AA, Sulaiman GM. "Anticancer activity and toxicity of carbon nanoparticles produced by pulsed laser ablation of graphite in water". Adv Nat Sci: Nanosci. Nanotechnol. 2020; 11(3): 035010.

Jabir M, Sahib UI, Taqi Z, Taha A, Sulaiman G, Albukhaty S. "Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line". Int J Nanomed. 2020; 15: 9025.

Sameen AM, Jabir MS, Al-Ani MQ. "Therapeutic combination of gold nanoparticles and LPS as cytotoxic and apoptosis inducer in breast cancer cells". AIP Conference Proceedings. 2020; 2213 (1): 020215.

Francis, S., Joseph, S., Koshy, E. P., and Mathew, B., "Green synthesis and characterization of gold and silver nanoparticles using Mussaenda glabrata leaf extract and their environmental applications to dye degradation", Environmental Science and Pollution Research. 2017; 24(21): 17347-17357.

Raj, S., Mali, S. C., and Trivedi, R., "Green synthesis and characterization of silver nanoparticles using enicostemma axillare (Lam.) leaf extract", Biochemical and biophysical research communications. 2018; 503(4): 2814-2819‏.

Abdallaha, B. F., Younus, M. A., & Ibraheem, I. J. "Preparation, Characterization, Antimicrobial and Antitumor Activity of Chitosan Schiff base/PVA/PVP Au, Ag Nanocomposite in Treatment of Breast Cancer Cell Line", Nanomed Res J. 2021; 6(4):369-384.

Rajeshkumar, S., "Anticancer activity of eco-friendly gold nanoparticles against lung and liver cancer cells", Journal of Genetic Engineering and Biotechnology. 2016; 14(1): 195-202‏.

Pokrowiecki, R., Wojnarowicz, J., Zareba, T., Koltsov, I., Lojkowski, W., Tyski, S., and Zawadzki, P., "Nanoparticles and human saliva: A step towards drug delivery systems for dental and craniofacial biomaterials", International journal of nanomedicine.2019; 14: 9235.

Damiri, F., Bachra, Y., Bounacir, C., Laaraibi, A., and Berrada, M., "Synthesis and characterization of lyophilized chitosan-based hydrogels cross-linked with benzaldehyde for controlled drug release", Journal of Chemistry (2020). ‏

Al Banna, L. S., Salem, N. M., Jaleel, G. A., and Awwad, A. M., "Green synthesis of sulfur nanoparticles using Rosmarinus officinalis leaves extract and nematicidal activity against meloidogyne javanica", Chemistry International. 2020; 6(3):137-143.

Yildirim, A., and Bulut, Y., "Adsorption behaviors of malachite green by using crosslinked chitosan / polyacrylic acid/bentonite composites with different ratios", Environmental Technology and Innovation. 2020; 17: 100560.

Hadidi, M., Pouramin, S., Adinepour, F., Haghani, S., and Jafari, S. M., "Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities", Carbohydrate polymers 2020; 236: 116075‏.

Nada H. Aljarba, Shah Imtiaz, Naushad Anwar, Ibtesam S. Alanazi, Saad Alkahtani,, " Anticancer and microbial activities of gold nanoparticles: A mechanistic review", Journal of King Saud University – Science. 2022; 34(4), 101907.

Firdhouse, J., & Lalitha, P. Apoptotic efficacy of biogenic silver nanoparticles on human breast cancer MCF-7 cell lines. Progress in biomaterials 2015; 4(2), 113-121.

Mohamed, N., "Synthesis of hybrid chitosan silver nanoparticles loaded with doxorubicin with promising anti-cancer activity". Bio Nano Science.2020; 10: 758-765

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Published

2024-06-29

Issue

Vol. 33 No. 2 (2024): Iraqi J Pharm Sci

Section

Article

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Copyright (c) 2024 Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)

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How to Cite

1.
Aldabbagh M, Baker F. Abdallaha, Nibras Y. Abdulla, Ibraheem J. Ibraheem. Synthesis, Characterization and Anticancer Activity of Chitosan Schiff Base / PEG Blend Doped with Gold and Silver Nanoparticles in Treatment of Breast Cancer Cell Line MCF-7. IJPS [Internet]. 2024 Jun. 29 [cited 2024 Oct. 18];33(2):101-1. Available from: https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/2548