Efficacy of Zingiber Officinale (ZO) in The Treatment of Lead Acetate-induced Hepatopathy in Rabbits: An Ultrastructural Assessment of Sub-acute Trial.

Efficacy of Zingiber Officinale (ZO) in the Treatment of Lead Acetate-Induced Hepatopathy in Rabbits


  • Mohammed Abdulabbas Hasan Department of Pathological Analysis, College of Applied Medical Scinces, Al-Shatrah University, Iraq. 2Department of Pathology, Faculty of Medicine, University of Thi-Qar, Iraq. 3Department of Pharmacology and Microbiology, Faculty of Biomedical Science, Universiti Putra Malaysia, Serdang, Malaysia.




Lead acetate, Hepatopathy, Ginger, SOD, Ultrastructural changes.


Lead acetate (Pb) is a hazardous heavy metal that is well-known to impair the functions of bodily systems and organs. This present study identifies the histopathological alterations that Pb toxicity causes in the liver of rabbits as well as the efficacy of ginger (Zingiber Officinale, ZO) in mitigating its negative effects. As ZO is a rich source of several antioxidants, this present study examines its ability to decrease Pb toxicity in test animals. A total of 30 rabbits were randomly divided into three groups (n = 10). The Control group was only fed distilled water, the Negative group was only fed a 2% Pb solution, while the Treated group was fed a 2% Pb solution as well as 100 mg of ZO extract per kilogram of body weight daily. Lead acetate (Pb) was found to significantly alter the histopathology of rabbit livers by increasing inflammation, fibrosis, vacuolation, and degeneration; significantly decreasing plasma superoxide dismutase (SOD) and catalase (CAT) enzymatic activities; and increasing plasma malondialdehyde (MDA) concentrations. Furthermore, ZO extract not only significantly decreases the negative hepatic impacts of Pb but also prevents Pb-induced hepatopathy.



Shalan MG, Mostafa MS, Hassouna MM, El-Nabi SEH, El-Refaie A. Amelioration of lead toxicity on rat liver with vitamin C and silymarin supplements. Toxicology. 2005;206(1):1–15.

Yücebilgiç G, Bilgin R, Tamer L, Tükel S. Effects of lead on Na+-K+ ATPase and Ca+ 2 ATPase activities and lipid peroxidation in blood of workers. Int J Toxicol. 2003;22(2):95–7.

Gidlow DA. Lead toxicity. Occup Med (Chic Ill). 2015;65(5):348–56.

Khalil-Manesh F, Tartaglia-Erler J, Gonick HC. Experimental model of lead nephropathy. IV. Correlation between renal functional changes and hematological indices of lead toxicity. J Trace Elem Electrolytes Health Dis. 1994;8(1):13–9.

Janovská Z. Bisphosphonate-related osteonecrosis of the jaws. A severe side effect of bisphosphonate therapy. Acta Medica (Hradec Kral. 2012;55(3):111–5.

Gurer-Orhan H, Sabır HU, Özgüneş H. Correlation between clinical indicators of lead poisoning and oxidative stress parameters in controls and lead-exposed workers. Toxicology. 2004;195(2–3):147–54.

Chaurasia SS, Kar A. Protective effects of vitamin E against lead-induced deterioration of membrane associated type-I iodothyronine 5′-monodeiodinase (5′ DI) activity in male mice. Toxicology. 1997;124(3):203–9.

Atwood CS, Huang X, Moir RD, Tanzi RE, Bush AI. Role of free radicals and metal ions in the pathogenesis of Alzheimer’s disease. Met Ions Biol Syst. 2018;309–64.

Pande M, Mehta A, Pant BP, Flora SJS. Combined administration of a chelating agent and an antioxidant in the prevention and treatment of acute lead intoxication in rats. Environ Toxicol Pharmacol. 2001;9(4):173–84.

Sandhir R, Gill KD. Effect of lead on lipid peroxidation in liver of rats. Biol Trace Elem Res. 1995;48:91–7.

Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic Biol Med. 2000;29(10):927–45.

Lykkesfeldt J, Svendsen O. Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J. 2007;173(3):502–11.

Alberti A, Bolognini L, Macciantelli D, Caratelli M. The radical cation of N, N-diethyl-para-phenylendiamine: a possible indicator of oxidative stress in biological samples. Res Chem Intermed. 2000;26:253–67.

Flora SJS, Pande M, Mehta A. Beneficial effect of combined administration of some naturally occurring antioxidants (vitamins) and thiol chelators in the treatment of chronic lead intoxication. Chem Biol Interact. 2003;145(3):267–80.

Chen CC, Kuo MC, Wu CM, Ho CT. Pungent compounds of ginger (Zingiber officinale Roscoe) extracted by liquid carbon dioxide. J Agric Food Chem. 1986;34(3):477–80.

Herrmann K. Antioxydativ wirksame Pflanzenphenole sowie Carotinoide als wichtige Inhaltsstoffe von Gewurzen. Gordian. 1994;94(7):113–6.

Masuda Y, Kikuzaki H, Hisamoto M, Nakatani N. Antioxidant properties of gingerol related compounds from ginger. Biofactors. 2004;21(1‐4):293–6.

Si W, Chen YP, Zhang J, Chen Z-Y, Chung HY. Antioxidant activities of ginger extract and its constituents toward lipids. Food Chem. 2018;239:1117–25.

Khaki AA, Khaki A. Antioxidant effect of ginger to prevents lead-induced liver tissue apoptosis in rat. J Med Plants Res. 2010;4(14):1492–5.

Ali BH, Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol. 2008;46(2):409–20.

Miller JK, Brzezinska-Slebodzinska E, Madsen FC. Oxidative stress, antioxidants, and animal function. J Dairy Sci. 1993;76(9):2812–23.

Wong MV, Khaw K-Y. Antioxidant, anti-cholinesterase and antibacterial activities of the bark extracts of Garcinia hombroniana. African J Pharm Pharmacol. 2013;7(8):454–9.

Nanyonga SK, Opoku A, Lewu FB, Oyedeji AO, Singh M. Chemical composition, antioxidant activity and cytotoxicity of the essential oils of the leaves and stem of Tarchonanthus camphoratus. African J Pharm Pharmacol. 2013;7(7):360–7.

Nicoll R, Henein MY. Ginger (Zingiber officinale Roscoe): a hot remedy for cardiovascular disease? Int J Cardiol. 2009;131(3):408–9.

GDOURA N, MURAT J-C, ABDELMOULEH A, ELFEKI A. Effects of Juniperus phoenicea extract on uricemia and activity of antioxidant enzymes in liver, erythrocyte and testis of hyperuricemic (oxonate-treated) rats. African J Pharm Pharmacol. 2013;7(8):416–25.

Khaki A, FATHI AF, Nouri M, Khaki AA, OZANCI CHC, GHAFARI NM, et al. The effects of Ginger on spermatogenesis and sperm parameters of rat. 2009;

Corrin B. Carleton’s histological technique. J Clin Pathol. 1981;34(5):572.

Attia AM, Ibrahim FA, Nabil GM, Aziz SW. Antioxidant effects of ginger (Zingiber officinale Roscoe) against lead acetate-induced hepatotoxicity in rats. Afr J Pharm Pharmacol. 2013;7(20):1213–9.

Abdou HM, Newairy AA. Hepatic and reproductive toxicity of lead in female rats and attenuation by flaxseed lignans. J Med Res Inst. 2006;27:295–302.

Suradkar SG, Vihol PD, Patel JH, Ghodasara DJ, Joshi BP, Prajapati KS. Patho-morphological changes in tissues of Wistar rats by exposure of Lead acetate. Vet world. 2010;3(2):82–4.

Patrick L. Lead Toxicity, a review of the literature. Part I: Exposure, Evaluation, and treatment. Altern Med Rev. 2006;11(1).

Al-Bideri AW. Histopathological study on the effect of antioxidants (vitamin E and selenium) in hepatotoxicity induced by lead acetate in rats. Qadisiah Med J. 2011;7:142–55.

Özcan MM. The effect of ginger (Zingiber officinale) powders at different concentrations on bioactive compounds, antioxidant activity, phenolic constituents, nutrients and sensory characteristics of wheat bread. Int J Gastron Food Sci. 2022;28:100532.

Joshi D, Srivastav SK, Belemkar S, Dixit VA. Zingiber officinale and 6-gingerol alleviate liver and kidney dysfunctions and oxidative stress induced by mercuric chloride in male rats: A protective approach. Biomed Pharmacother. 2017;91:645–55.

Patora RC, Swarup D. Effect of antioxidant ascorbic acid, 1–methionine or & tocopherol alone or along with chelator on cardiac tissue of lead–treated rats. Veterinarhiv. 2004;74(3):235–44.

Upasani CD, Khera A, Balararnan R. Effect of lead with vitamin E, C, or Spirulina on malondialdehyde, conjugated dienes and hydroperoxides in rats. 2001;

Foster WG. Reproductive toxicity of chronic lead exposure in the female cynomolgus monkey. Reprod Toxicol. 1992;6(2):123–31.

38. Lisa T. The top 10 Antioxidant foods. Better Nutr Available from http//www crumcreek com/library/antioxidant html. 2002;

Mustafa I, Chin NL. Antioxidant Properties of Dried Ginger (Zingiber officinale Roscoe) var. Bentong. Foods. 2023;12(1):178.