Isolation, Structural Characterisation and Wound Healing Activity of Flavonoid and its Glycoside from Euphorbia hirta L. Methanolic Extract
DOI:
https://doi.org/10.31351/vol33iss3pp89-105Keywords:
Wound healing, Euphorbia hirta, Euphorbiaceae, kaempferol, astragalinAbstract
Euphorbia hirta is a plant belonging to the Euphorbiaceae family that is widely used in traditional medicine to treat various dermal diseases in tropical and subtropical countries. It has been found to have anti-inflammatory and wound-healing effects, which may make it a promising natural treatment for different medical conditions. The aim of the present study focuses on the investigation of the main active compounds associated with the wound-healing activity of plants. The plant powder of aerial part was sequentially extracted with n-hexane, chloroform, methanol, and water using the serial exhaustive extraction method.The extract with the best activities was further fractionated using various chromatographic techniques to isolate the active compounds responsible for wound healing. The isolated compounds were assessed for wound healing activity by measuring migration and proliferation against human dermal fibroblast cells. The structure elucidation of compounds was confirmed by IR, LC-MS, and NMR. These isolated compounds were subsequently identified as kaempferol and its glycoside, kaempferol-3-O-glucoside (astragalin). The findings also revealed that both isolated compounds were able to stimulate the cell population with no sign of toxicity and the highest cell migration ability shows after 48-hour treatment. This study provides new evidence to support the traditional use of E. hirta as a natural promising wound healer.
Received 8/6/2023
Accepted 4/9/2023
Published 15/9/2024
How to Cite
Publication Dates
References
Salehi B, Iriti M, Vitalini S, Antolak H, Pawlikowska E, Kręgiel D, et al. Euphorbia-Derived Natural Products with Potential for Use in Health Maintenance. Biomolecules. 2019;9(8):337.
Kumar S, Malhotra R, Kumar D. Euphorbia hirta: Its chemistry, traditional and medicinal uses, and pharmacological activities. Pharmacogn Rev. 2010;4(7):58-61.
Al-Snafi AE. Pharmacology and therapeutic potential of Euphorbia hirta (Syn: Euphorbia pilulifera)- A review. IOSRPHR IOSR Journal of Pharmacy (IOSRPHR). 2017;07(03):07-20.
Shenoy C, Patil MB, Kumar R, Patil S. Preliminary phytochemical investigation and wound healing activity of Allium cepa linn (Liliaceae). Int J Pharmcy Pharm Sci International Journal of Pharmacy and Pharmaceutical Sciences. 2009(2):167-75.
Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. Journal of international medical research. 2009;37(5):1528-42.
Guo S, DiPietro LA. Factors Affecting Wound Healing. J Dent Res Journal of Dental Research. 2010;89(3):219-29.
Brown A. The principles of holistic wound assessment. Nur Times Nursing Times. 2015;111(46):14-6.
Schmidt C, Fronza M, Goettert M, Geller F, Luik S, Flores E, et al. Biological studies on Brazilian plants used in wound healing. Journal of ethnopharmacology. 2009;122:523-32.
Meher B, Satapathy T, Sahu AK, Ahirwar K, Kashinath PD, Jain NP. Screening of methanolic extract of euphorbia hirta linn for antiinflammatory activity in experimental animals. Research Journal of Pharmacy and Technology. 2012;5:38-40.
Rana S, Kumer A, Rahman SM. Antithrombotic and anti-inflammatory activities of leaf methanolic extract of Euphorbia hirta Lin. Int J Complement Alt Med. 2019;12:154-62.
Addis R, Cruciani S, Santaniello S, Bellu E, Sarais G, Ventura C, et al. Fibroblast proliferation and migration in wound healing by phytochemicals: Evidence for a novel synergic outcome. 2020;17(8):1030-42.
Telange DR, Patil AT, Pethe AM, Tatode AA, Anand S, Dave VS. Kaempferol-phospholipid complex: formulation, and evaluation of improved solubility, in vivo bioavailability, and antioxidant potential of kaempferol. Journal of Excipients and Food Chemicals. 2016;7(4):1174.
Yi X, Zuo J, Tan C, Xian S, Luo C, Chen S, et al. Kaempferol, a flavonoid compound from gynura medica induced apoptosis and growth inhibition in mcf-7 breast cancer cell. frican Journal of Traditional, Complementary Alternative Medicines. 2016;13(4):210-5.
Fawzi F, Mahdi MF, Abaas IS. Isolation of Astragalin from Cressa cretica cultivated in Iraq. J Pharm Sci & Res. 2019;11(1):185-90.
Ghavam-Haghi F, Sadeghi Dinani M. Isolation and identification of Astragalin and 2-methoxy tyrosol from the bulbs of Allium paradoxum. Journal of HerbMed Pharmacology. 2017;6(3):114-8.
Mehdi A, Al-ani WM, Raoof A. Isolation of astragalin from iraqi Chenopodium album. Asian Journal of Pharmaceutical and Clinical Research. 2018;11:530.
Alsaffar DF, Yaseen A, Mahmud R, Aziz NHKA. Wound Healing Studies of Selected Euphorbia Species: A Review. Annals of RSCB 2021;25(6):15542-55.
Fenu M, Bettermann T, Vogl C, Darwish-Miranda N, Schramel J, Jenner F, et al. A novel magnet-based scratch method for standardisation of wound-healing assays. Scientific reports. 2019;9(1):1-9.
Suktap C, Lee HK, Amnuaypol S, Suttisri R, Sukrong S. Wound Healing Effect of Flavonoid Glycosides from Afgekia mahidolae BL Burtt & Chermsir. Leaves. Records of Natural Products. 2018;12(4):391-6.
Aslam M, Riaz H, Raza SA, Hussain S, Qureshi O, Hamzah Z, et al. Role of Flavonoids as Wound Healing Agent. 2018. p. 95-102.
Kim JK, Park SU. Recent studies on kaempferol and its biological and pharmacological activities. EXCLI J. 2020;19:627-34.
Ghosh PK, Gaba A. Phyto-extracts in wound healing. J Pharm Pharm Sci. 2013;16(5):760-820.
Özay Y, Güzel S, Yumrutaş Ö, Pehlivanoğlu B, Erdoğdu İH, Yildirim Z, et al. Wound healing effect of kaempferol in diabetic and nondiabetic rats. J Surg Res. 2019;233:284-96.
Riaz A, Rasul A, Hussain G, Zahoor MK, Jabeen F, Subhani Z, et al. Astragalin: a bioactive phytochemical with potential therapeutic activities. Advances in Pharmacological Sciences. 2018;2018:1-15.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)
This work is licensed under a Creative Commons Attribution 4.0 International License.
