Investigation of the relationship between adiponectin gene polymorphism (ADIPOQ SNP rs-266729) and obesity Patients / Basra-Iraqi
DOI:
https://doi.org/10.31351/vol33iss3pp30-36Keywords:
ADIPOQ gene polymorphism, BMI, Obesity, and SNP (rs266729)Abstract
Adiponectin is one of the most important hormones secreted by adipose tissue and plays a major role in the development of obesity. A variant-specific ADIPOQ single nucleotide polymorphism (SNP rs-266729) with a C to G missense mutation can be strongly associated with obesity. Therefore, this study aims to evaluate the association of single nucleotide polymorphisms (SNP rs-266729) of the ADIPOQ gene with obesity and some biochemical markers in these patients. The case-control study included 186 participants (106 patients and 80 healthy control). DNA was extracted from whole blood and then the Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP-PCR) technique was used to limit the genotype of the ADIPOQ gene (rs266729) polymorphism. Also, the lipid profile, glycated hemoglobin (HbA1C), fasting blood glucose (FBG), insulin, and adiponectin were measured by standard methods. Results: The allele G in the ADIPOQ-gene polymorphism (rs266729) was a significantly higher frequency of 23 (21.6%) in the obese patients (P_Value = 0.039) compared with the control subject of 10 (12.5%). It significantly increased the risk in the additive model and allele frequency (P = 0.0139 and 0.0133), respectively. The rs266729 SNP showed a significant association with increased BMI, insulin, HOMO-IR, TG, VLDL, and lower adiponectin at a p-value of 0.001 for all, after adjustments for age and sex. Lower levels of high-density lipoprotein (HDL) were observed in the CG and GG genotypes compared to the ADIPOQ rs266729 SNP C.C genotype (p = 0.032). In obese individuals, there was no significant relationship between rs266729 SNP and HbA1C, FBS, TC, and LDL. In conclusion, the single nucleotide polymorphism (rs266729) of the ADIPOQ gene has a significant difference with BMI, insulin, TG, VLDL, and HOMO-IR in obese patients, which might be the cause of obesity.
Received 15/6/2023
Accepted 2/8/2023
Published 15/9/2024
References
Carrillo JLM, Campo JOMD, Coronado OG, Gutiérrez PTV, Cordero JFC, Juárez JV. Adipose Tissue and Inflammation [Internet]. Adipose Tissue. InTech; 2018. Available from: http://dx.doi.org/10.5772/intechopen.74227.
Martinez-Huenchullan SF, Tam CS, Ban LA, Ehrenfeld-Slater P, Mclennan S V., Twigg SM. Skeletal muscle adiponectin induction in obesity and exercise. Metabolism. 2020; 102:154008.
Khoramipour K, Chamari K, Hekmatikar AA, Ziyaiyan A, Taherkhani S, Elguindy NM, et al. Adiponectin: Structure, Physiological Functions, Role in Diseases, and Effects of Nutrition. 2021;1–15.
Melo J, Ribeiro AI, Aznar S, Pizarro A, Santos MP. Urban green spaces, greenness exposure and species richness in residential environments and relations with physical activity and bmi in portuguese adolescents. Int J Environ Res Public Health. 2021;18(12).
De Luis DA, Primo D, Izaola O, Aller R. Adiponectin Gene Variant rs266729 Interacts with Different Macronutrient Distribution of Two Different Hypocaloric Diets. Lifestyle Genomics. 2020;13(1):20–7.
Parida, S., Siddharth, S., & Sharma, D. Adiponectin, obesity, and cancer: clash of the bigwigs in health and disease. International journal of molecular sciences. 2019;20 (10), 2519
Tumminia A, Vinciguerra F, Parisi M, Graziano M, Sciacca L, Baratta R, et al. Adipose tissue, obesity and adiponectin: Role in endocrine cancer risk. Int J Mol Sci. 2019;20(12).
Rizza S, Gigli F, Galli A, Micchelini B, Lauro D, Lauro R, et al. Adiponectin isoforms in elderly patients with or without coronary artery disease. J Am Geriatr Soc. 2010;58(4):702–6.
Sun P, Liu L, Chen J, Chen Y, Shi L, Imam MU, et al. The polymorphism of rs266729 in adiponectin gene and type 2 diabetes mellitus: A Meta-Analysis. Med (United States). 2017;96(47).
Bogner, B., Schwenoha, K., Vogl, M., Weghuber, D., Roth, C., Kipman, U., ... & Oostingh, G. J. Evaluation of reference intervals of haematological and biochemical markers in an Austrian adolescent study cohort. Clinical Chemistry and Laboratory Medicine (CCLM), 2019; (57) 6, 891-900.
Bouzid, K., Bahlous, A., Ferjani, W., Kalai, E., Ducrocq, R., Ben Mami, F., & Abdelmoula, J, et al. Advantage of HbA1c assay by HPLC D-10 versus cobas integra 400 in a population carrier for HbS and HbC. Clinical Laboratory, 2012; 58(7), 821.
Madlu, M. G., & Abood, W. S. Genotyping of Human Papilloma Virus Causing Skin Wart among Iraqi Patients. Indian Journal of Forensic Medicine & Toxicology, 2021, 15.2.
Polska Tom E, Puzianowska-Kuźnicka M, Roszkowska-Gancarz M, Bartoszewicz Z, Polosak J, Kurylowicz A, et al. Prace oryginalne/original PaPers Total and high molecular weight adiponectin and level--modifying polymorphisms of ADIPOQ in centenarians Całkowita i wysokocząsteczkowa adiponektyna oraz modyfikujące stężenie adiponektyny polimorfizmy genu ADIPOQ u stulat. Endokrynol Pol. 2012;63(6):439–46.
Alharbi MS, Khabour OF, Alomari MA. The association between adiponectin plasma level and rs1501299 ADIPOQ polymorphism with atrial fibrillation. J King Saud Univ - Sci. 2023;35(4):102655.
Suriyaprom K, Phonrat B, Tungtrongchitr R. Association of adiponectin gene -11377C>G polymorphism with adiponectin levels and the metabolic syndrome in Thais. Asia Pac J Clin Nutr. 2014;23(1):167–73.
Elghazy AM, Elsaeid AM, Refaat M, Youssef MM. Biochemical studies of adiponectin gene polymorphism in patients with obesity in Egyptians. Arch Physiol Biochem. 2022;128(1):43–50.
Kim AY, Park YJ, Pan X, Shin KC, Kwak SH, Bassas AF, et al. Obesity-induced DNA hypermethylation of the adiponectin gene mediates insulin resistance. Nat Commun. 2015;6(May):1–11.
Wu N, Zhai X, Yuan F, Li J, Li D, Wang J, et al. Genetic variation in TBC1 domain family member 1 gene associates with the risk of lean NAFLD via high-density lipoprotein. Front Genet. 2023;13(January):1–10.
Cheng J, Li Y, He Q, Luo L, Zhang Y, Gao Y. Essential hypertension in patients exposed to high-arsenic exposed areas in western China: Genetic susceptibility and urinary arsenic metabolism characteristics. J Trace Elem Med Biol. 2021;67(May):126778.
De Luis DA, Izaola O, Primo D, Aller R. Relation of a variant in adiponectin gene (rs266729) with metabolic syndrome and diabetes mellitus type 2 in adult obese subjects. Eur Rev Med Pharmacol Sci. 2020;24(20):10646–52.
Fagian Pansani V, et al. Adiponectin: A “Friendly adipokine” in Diabetic Retinopathy? In: Seminars in Ophthalmology. Taylor & Francis, 2023. p. 1-8.
Leońska-Duniec A, Grzywacz A, Jastrzȩbski Z, Jazdzewska A, Lulińska-Kuklik E, Moska W, et al. ADIPOQ polymorphisms are associated with changes in obesity-related traits in response to aerobic training programme in women. Biol Sport. 2018;35(2):165–73.
Karachentsev Y, Gorshunska M, Tyzhnenko T, Krasova N, Dunaeva I, Gladkih A, et al. Однонуклеотидні Поліморфізми Гена Адипонектину У Хворих На Цукровий Діабет 2-Го Типу Та Неалкогольну Жирову Хворобу Печінки. Int J Endocrinol. 2021;13(4):229–37.
Hussein SEO, Altoum AEA, Osman AL, Higazi HMKI, Ali S, Alfeel AH. Influence of BMI on Serum Adiponectin, Resistine, and FBG among Overweight and Obese Females Diabetic Patient Type2. Open Access Maced J Med Sci. 2022;10(B):1218–21.
Sitar-Tǎut AV, Cozma A, Fodor A, Coste SC, Orasan OH, Negrean V, et al. New insights on the relationship between leptin, ghrelin, and leptin/ghrelin ratio enforced by body mass index in obesity and diabetes. Biomedicines. 2021;9(11).
Katta N, Loethen T, Lavie CJ, Alpert MA. Obesity and Coronary Heart Disease: Epidemiology, Pathology, and Coronary Artery Imaging. Curr Probl Cardiol. 2021;46(3):100655.
Jahangir E, De Schutter A, Lavie CJ. The relationship between obesity and coronary artery disease. Transl Res. 2014;164(4):336–44.
Hussain ZK. Study of Possible Changes in Lipid Profiles between Premenopausal and Postmenopausal Women with Hyperthyroidism and Others with Hypothyroidism. Iraqi J Sci. 2022;63(12):5139–46.
Rajendra S, Nalini R, Aravinthan M. Prevalence of Obesity, non-alcoholic fatty liver and insulin resistance and them inter relationship among type II diabetic patients attending diabetic centre, Teaching Hospital, Jaffna. Jaffna Med J. 2020;31(2): 26.
Hashim MF, Ail DS, Issa AY. Evaluation of some Biochemical parameters in serum patients with thyroid gland dysfunction. Basrah J Sci. 2018;36 C (1):42–51.
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