Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats
DOI:
https://doi.org/10.31351/vol34iss1pp72-84Keywords:
boswellic acid, epithelialization, excision wound, wound healingAbstract
Wound is known as one of the serious health issue. Boswellia serrata (BS) contains a wide range of phytochemicals with different pharmacological properties. The study aimed to assess the wound healing potential of Boswellia Serrata Extract (BSE) against an experimental model of excision wound. A total of 45 rats in five equal groups were used in this investigation. The wound was created in the cervical dorsal region of each rat as a two-symmetrical circle pattern. All groups were being treated as follow: control group with ointment base, β-Sitosterol group with 0.25% w/w β-Sitosterol-containing ointment, three BSE ointment groups; treated with BSE ointment containing 5%, 10%, and 20% boswellic acid (BA) respectively. The wounds were followed up for 15 days. On days 5, 10, and 15, three rats from each group were sacrificed, the wounds and scars were sent for histopathological study. The ointments containing 5% and 10% BA exerted a significantly increased wound contraction rate, lesser surface area and shorter re-epithelialization period in the macroscopical analysis in compare to the control group and the β-Sitosterol group. However, the microscopical results exhibited a significant healing progress in a concentration dependent-manner. This was reflected by formation of clear dermal granulation tissue, collagen deposition and angiogenesis in quantitative histological evaluation. In conclusion, this study provided a macroscopical and microscopical evidences for a potential wound healing effect of the topical application of BSE in concentration dependent manner. Although the highest BA concentration in macroscopical analysis exhibited lesser efficacy.
How to Cite
Publication Dates
Received
Revised
Accepted
Published Online First
References
Boateng JS, Matthews KH, Stevens HNE, Eccleston GM. Wound Healing Dressings and Drug Delivery Systems: A Review. J Pharm Sci. 2008;97(8):2892–923.
Demidova-Rice TN, Hamblin MR, Herman IM. Acute and Impaired Wound Healing: Pathophysiology and Current Methods for Drug Delivery, Part 1 Normal and Chronic Wounds Biology, Causes, and Approaches to Care. Adv Skin Wound Care. 2012;25(7):304–14.
Shenoy RR, Sudheendra AT, Nayak PG, Paul P, Kutty NG, Rao CM. Normal and delayed wound healing is improved by sesamol, an active constituent of Sesamum indicum (L.) in albino rats. J Ethnopharmacol [Internet]. 2011;133(2):608–12.
Yang Y, Wang F, Yin D, Fang Z, Huang L. Astragulus polysaccharide-loaded fibrous mats promote the restoration of microcirculation in/around skin wounds to accelerate wound healing in a diabetic rat model. Colloids Surf B Biointerfaces. 2015;136:111–8.
Xu F, Wu J, Hu Y, Chu C, Liu W, Li X, et al. Mechanisms of action underlying the effect of Tongsaimai on wound healing based on experimental and network pharmacology. J Ethnopharmacol. 2023;116451.
Albahri G, Badran A, Hijazi A, Daou A, Baydoun E, Nasser M, et al. The Therapeutic Wound Healing Bioactivities of Various Medicinal Plants. Life. 2023;13(2):317.
Hamidpour R, Hamidpour S, Hamidpour M, Shahlari M. Frankincense (乳香 Rǔ Xiāng; Boswellia Species): From the Selection of Traditional Applications to the Novel Phytotherapy for the Prevention and Treatment of Serious Diseases. J Tradit Complement Med. 2013 Oct;3(4):221–6.
Iram F, Khan SA, Husain A. Phytochemistry and potential therapeutic actions of Boswellic acids: A mini-review. Asian Pac J Trop Biomed. 2017;7(6):513–23.
Siddiqui MZ. Boswellia serrata, a potential antiinflammatory agent: an overview. Indian J Pharm Sci. 2011;73(3):255–61.
Roy NK, Parama D, Banik K, Bordoloi D, Devi AK, Thakur KK, et al. An Update on Pharmacological Potential of Boswellic Acids against Chronic Diseases. Int J Mol Sci. 2019 Aug 22;20(17):E4101.
Pengzong Z, Yuanmin L, Xiaoming X, Shang D, Wei X, Zhigang L, et al. Wound Healing Potential of the Standardized Extract of Boswellia serrata on Experimental Diabetic Foot Ulcer via Inhibition of Inflammatory, Angiogenetic and Apoptotic Markers. Planta Med. 2019;85(08):657–69.
Sahinfard N, Namjoo A, Arami R, Rafieian M, Baradaran A, Nasri H, et al. Remedial Effect of Boswellia Serrata on Thermal Burn Injuries. Shiraz E-Med J. 2015;16(1).
Allen LV, Ansel HC. Ansel’s pharmaceutical dosage forms and drug delivery systems. Tenth edition. Philadelphia: Wolters Kluwer Health; 2014. 794 p.
Caetano GF, Frade MAC, Andrade TAM, Leite MN, Bueno CZ, Moraes ÂM, et al. Chitosan-alginate membranes accelerate wound healing: CHITOSAN-ALGINATE MEMBRANES ACCELERATE WOUND HEALING. J Biomed Mater Res B Appl Biomater. 2015;103(5):1013–22.
Masson‐Meyers DS, Andrade TAM, Caetano GF, Guimaraes FR, Leite MN, Leite SN, et al. Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol. 2020;101(1–2):21–37.
Jewo PI, Fadeyibi IO, Babalola OS, Saalu LC, Benebo AS, Izegbu MC, et al. A Comparative Study of the Wound Healing Properties of Moist Exposed Burn Ointment (MEBO) and Silver Sulphadiazine. Ann Burns Fire Disasters. 2009;22(2):79–82.
Okur ME, Ayla Ş, Çiçek Polat D, Günal MY, Yoltaş A, Biçeroğlu Ö. Novel insight into wound healing properties of methanol extract of Capparis ovata Desf. var. palaestina Zohary fruits. J Pharm Pharmacol [Internet]. 2018;70(10):1401–13.
Wang J ping, Ruan J lan, Cai Y ling, Luo Q, Xu H xing, Wu Y xia. In vitro and in vivo evaluation of the wound healing properties of Siegesbeckia pubescens. J Ethnopharmacol. 2011;134(3):1033–8.
Dash GK, Murthy PN. Studies on Wound Healing Activity of Heliotropium indicum Linn. Leaves on Rats. ISRN Pharmacol. 2011;2011:1–8.
Maraghehpour B, Khayamzadeh M, Najafi S, Kharazifard M. Traditionally used herbal medicines with antibacterial effect on Aggegatibacter actinomycetemcomitans: Boswellia serrata and Nigella sativa. J Indian Soc Periodontol. 2016;20(6):603–7.
Beghelli D, Isani G, Roncada P, Andreani G, Bistoni O, Bertocchi M, et al. Antioxidant and Ex Vivo Immune System Regulatory Properties of Boswellia serrata Extracts. Oxid Med Cell Longev. 2017;2017:1–10.
Sharma ML, Bani S, Singh GB. Anti-arthritic activity of boswellic acids in bovine serum albumin (BSA)-induced arthritis. Int J Immunopharmacol. 1989;11(6):647–52.
Wagner H. Search for New Plant Constituents with Potential Antiphlogistic and Antiallergic Activity. Planta Med. 1989;55(03):235–41.
Alluri VK, Kundimi S, Sengupta K, Golakoti T, Kilari EK. An Anti-Inflammatory Composition of Boswellia serrata Resin Extracts Alleviates Pain and Protects Cartilage in Monoiodoacetate-Induced Osteoarthritis in Rats. Evid Based Complement Alternat Med. 2020;2020:1–12.
Cuaz-Pérolin C, Billiet L, Baugé E, Copin C, Scott-Algara D, Genze F, et al. Antiinflammatory and Antiatherogenic Effects of the NF-κB Inhibitor Acetyl-11-Keto-β-Boswellic Acid in LPS-Challenged ApoE −/− Mice. Arterioscler Thromb Vasc Biol. 2008;28(2):272–7.
Gilbert NC, Gerstmeier J, Schexnaydre EE, Börner F, Garscha U, Neau DB, et al. Structural and mechanistic insights into 5-lipoxygenase inhibition by natural products. Nat Chem Biol. 2020;16(7):783–90.
Ammon HPT. Boswellic Acids and Their Role in Chronic Inflammatory Diseases. In: Gupta SC, Prasad S, Aggarwal BB, editors. Anti-inflammatory Nutraceuticals and Chronic Diseases. Cham: Springer International Publishing; 2016. p. 291–327. (Advances in Experimental Medicine and Biology; vol. 928).
Sinno H, Prakash S. Complements and the Wound Healing Cascade: An Updated Review. Plast Surg Int. 2013 Jul 24;2013:1–7.
Bertocchi M, Isani G, Medici F, Andreani G, Tubon Usca I, Roncada P, et al. Anti-Inflammatory Activity of Boswellia serrata Extracts: An In Vitro Study on Porcine Aortic Endothelial Cells. Oxid Med Cell Longev. 2018;2018:1–9.
Ammon H. Boswellic Acids in Chronic Inflammatory Diseases. Planta Med. 2006;72(12):1100–16.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Iraqi Journal of Pharmaceutical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
