Antileishmanial Evaluation and Phytochemical Screening of Iraqi Sanchezia speciosa Petroleum Ether Fraction in vitro

Authors

  • Muntadher Salim Rasool Ministry of Health, Karbala Health Department, Karbala ,Iraq
  • Thukaa Z.Abdul-Jalil Department of Pharmacognosy and Medicinal Plants, College of Pharmacy ,University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.31351/vol34iss1pp266-274

Keywords:

Leishmania tropica, Leishmania donovani, Maceration, Pentostam, Sanchezia speciosa

Abstract

Leishmaniasis is a significant parasite issue. Vaccine inaccessibility and the absence of a safe and effective therapy that is not resistant to the illness hinders the total eradication of this disease and remains a challenge for the control of the disease; medications with harmful side effects or insufficient efficacy have resulted in disease recurrence.

The path towards discovering alternative medications derived from natural resources with shown antileishmanial activity has been paved using these criteria. Consequently, two Leishmanial parasites were examined for their antileishmanial activity, and an Iraqi plant was chosen. The anti-leishmania fraction activity was obtained from the aerial portions of Sanchezia speciosa, which has been assessed using in vitro methods.

Maceration of the aerial portions of Sanchezia speciosa with petroleum ether was used to create the extract, which included active non-polar compound components. To ensure accuracy, an MTT assay was utilized and the extract was prepared as six distinct concentrations with 1% DMSO, the medium with parasite serving as negative controls and Pentostam ( Sodium stibogluconate ) as positive controls. Leishmania donovani and Leishmania tropica were both tested for their ability to be effectively inhibited by the fraction that was obtained. Based on the extract of the plants at many doses tested, the results showed superior antileishmanial activity of macerated petroleum ether fraction with IC50 value (0.092 mg/ml) against Leishmania tropica and IC50 value (0.074 mg/ml) against Leishmania donovani when combined with the antileishmanial activity of the official treatment medication (Pentostam®); the chemical examination of the extracts revealed the presence of terpenes and steroids by qualitative phytochemical tests. The findings of this study indicate that the presence of certain secondary metabolites, such as terpene and steroid compounds in the materials utilized to make up this fraction likely explains this high potential antileishmanial action against Leishmania tropica and Leishmania donovani. Ongoing efforts are being made to find a successful approach for treating leishmaniasis with minimal side effects, and there is a significant focus on herbal medications.

How to Cite

1.
Muntadher Salim Rasool, Abdul-Jaleel TZ. Antileishmanial Evaluation and Phytochemical Screening of Iraqi Sanchezia speciosa Petroleum Ether Fraction in vitro. Iraqi Journal of Pharmaceutical Sciences [Internet]. 2025 Mar. 29 [cited 2025 Apr. 1];34(1):266-74. Available from: https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/3866

Publication Dates

Received

2024-06-01

Revised

2024-07-19

Accepted

2024-10-02

Published Online First

2025-03-29

References

Zuhair, T., Majeed Shah, S., & Abdul Jalil, T. Z. (n.d.). Investigation the Activity of Iraqi Agave attenuata on In Vitro Growth of Cutaneous Leishmania Promastigotes. In In Vitro Growth of Cutaneous Leishmania Promastigotes (Vol. 2023, Issue 01).

Badhwar S, Selvapandiyan A, Puri N. Essentials in Leishmaniasis Elimination. InChallenges and Solutions Against Visceral Leishmaniasis 2024 Jan 28 (pp. 43-62). Singapore: Springer Nature Singapore.

Shah NA, Khan MR, Nadhman A. Antileishmanial, toxicity, and phytochemical evaluation of medicinal plants collected from Pakistan. BioMed research international. 2014 Oct;2014.

SosaN, PascaleJM, Jime ´nez AI, NorwoodJA, Kreishman-Detrick M, Weina PJ, et al. Topicalparomo mycin for New World cutaneous leishmaniasis. PLoS neglected Tropical Diseases. 2019;13(5): e0007253.

CantanhêdeLM, FernandesFG, Ferreira GEM, PorrozziR, Ferreira RdGM, Cupolillo E. New insights into the genetic diversity of Leishmania RNA Virus 1 and its species-specific relationship with Leish mania parasites. PLOSONE.2018;13(6):e0198727.

Heidari-Kharaji M, Taheri T, Doroud D, Habibzadeh S, Rafati S. Solid lipid nanoparticle loaded with paromomycin: invivo efficacy against Leishmania tropica infection in BALB/c mice model. Appl Microbiol Biotechnol. 2016;100(16):7051–60.

Camacho MR, Phillipson JD, Croft SL, Solis PN, Marshall SJ, Ghazanfar SA. Screening of plant extracts for antiprotozoal and cytotoxic activities. J Ethnopharmacol. 2003;89(2-3):185–91.

Leonard EC and Smith LB. Sanchezia and related American Acanthaceae. Rhodora1964; 66: 313-343.

Ahmed ZU. Encyclopedia of Flora and Fauna of Bangladesh.1st ed. Vol 6, Dhaka, Nimtali: Asiatic Society of Bangladesh; 2008, p. 59.

Bui Thanh T, Vu Duc L, Nguyen Thanh H, Nguyen Tien V. In vitro antioxidant and anti-inflammatory activities of isolated compounds of ethanol extract from Sanchezia speciosa Leonard’s leaves. Journal of basic and clinical physiology and pharmacology. 2017 Jan 1;28(1):79-84.

Rafshanjani MA, Parvin S, Kader MA, Sharmin T. Preliminary phytochemical screening and cytotoxic potentials from leaves of Sanchezia speciosa Hook. f. International Journal of Advances in Scientific Research. 2015 Apr 30;1(03):145-50.

Rafshanjani MA, Parvin S, Kader MA, Saha MR, Akhtar MA. In vitro antibacterial, antifungal and insecticidal activities of ethanolic extract and its fractionates of Sanchezia speciosa Hook. F. Int Res J Pharm 2014;5:717–20.

Paydar M, Wong YL, Moharam BA, Wong WF, Looi CY. In vitro anti-oxidant and anti-cancer activity of methanolic extract from Sanchezia speciosa leaves. Pak J Biol Sci 2013;16:1212.

Bui Thanh T, Vu Duc L, Nguyen Thanh H, Nguyen Tien V. In vitro antioxidant and anti-inflammatory activities of isolated compounds of ethanol extract from Sanchezia speciosa Leonard’s leaves. Journal of basic and clinical physiology and pharmacology. 2017 Jan 1;28(1):79-84.

Yang W, Chen X, Li Y, Guo S, Wang Z, Yu X. Advances in pharmacological activities of terpenoids. Natural Product Communications. 2020 Mar;15(3):1934578X20903555.===

Raimundo VD, Carvalho RP, Machado-Neves M, de Almeida Marques-da-Silva E. Effects of terpenes in the treatment of visceral leishmaniasis: A systematic review of preclinical evidence. Pharmacological Research. 2022 Mar 1;177:106117.===

Raimundo VD, Carvalho RP, Machado-Neves M, de Almeida Marques-da-Silva E. Effects of terpenes in the treatment of visceral leishmaniasis: A systematic review of preclinical evidence. Pharmacological Research. 2022 Mar 1;177:106117.===

Ali M, Kamal A, Rashid H, et al. Anti-leishmanial activity of phytosterols: β-sitosterol and stigmasterol. Phytotherapy Research. 2015;29(12):1923-1930.===

Araujo C, de Andrade-Neto J, Lima A, et al. Phytosterols and their effects on Leishmania parasites. Journal of Ethnopharmacology. 2021;275:114102.

Abdulqader, D. H., Al-Zubaydi, S. R., & Kadhim, E. J. (n.d.). International Journal of Pharmacy and Pharmaceutical Studies Isolation of Beta-Sitosterol, Campesterol and Stigmasterol from Alcea Kurdica Roots Petroleum Extract Using Preparative HPLC 1. 7.

Farhan MS, Khamees AH, Ahmed OH, AmerTawfeeq A, Yaseen YS. GC/MS analysis of n-hexane and chloroform extracts of Chenopodium murale leaves in Iraq. J. Pharm. Res. Int. 2019;31(6):1-6.

Khadim EJ, Abdulrasool AA, Awad ZJ. Phytochemical investigation of alkaloids in the Iraqi Echinops heterophyllus (Compositae). Iraqi J Pharm Sci. 2014;23:26–34.

Hussien MS, Al-Hamashi AA. Phytosterol Profile in Iraqi Lactuca serriola after Purification and Isolation by Combiflash and HPLC (Conference Paper). Iraqi Journal of Pharmaceutical Sciences (P-ISSN 1683-3597 E-ISSN 2521-3512). 2022;31(Suppl.):54-61.

Khalaf, R. A., & Abdul-Jalill, T. Z. Phytochemical Screening by GC/MS with Isolation and Characterization of ß-sitosterol and Stigmasterol from Iraqi Euonymus japonicus L. Leaves Parts by Different Techniques. International Journal of Drug Delivery Technology 2023; 13(1), 407–413.

Bansal D, Sehgal R, Chawla Y, et al. In vitro activity of anti-amoebic drugs against clinical isolates of Entamoeba histolytica and Entamoeba dispar. AnnClin Microbiol Antimicrob 2004; 3:1–5.

Sereno D, Lemesre JL. Axenically cultured amastigote forms as an in vitro model for investigation of antileishmanial agents. Antimicrob Agents Chemother 1997; 41:972–976.

Jaafar, N. S., Alshammaa, D. A., Abdul-Jalil, T. Z., & Ibrahem, N. M. (2022). Quantitative Determination and Cytotoxic Effect of Oleanolic Acid from Olea europaea Leaves Extract Cultivated in Iraq. Iraqi Journal of Pharmaceutical Sciences, 31(2), 244–250.

Al-Ogaili N. Synergistic effect of Lawsonia inermis and Peganum harmala aqueous extracts on in vitro growth of Leishmania tropica promastigotes comparison to sodium stibogluconate. Al-Qadisiyah Med J 2016; 12:76-83.

Jewely HM, Zuhair T. Evaluation of Antileishmanial Activity of Osteospermum ecklonis Extract of Aerial Parts against Leishmania donovani: in vitro (Conference Paper). Iraqi Journal of Pharmaceutical Sciences (P-ISSN 1683-3597 E-ISSN 2521-3512). 2022;31(Suppl.):45-53.

Aykul S, Martinez-Hackert E. Determination of half-maximal inhibitory concentration using biosensor-based protein interaction analysis. Analytical biochemistry. 2016 Jul 1;508:97-103.

Sebaugh JL. Guidelines for accurate EC50/IC50 estimation. Pharmaceutical statistics. 2011 Mar;10(2):128-34.

Gontijo VS, Espuri PF, Alves RB, et al. Leishmanicidal, antiproteolytic and mutagenic evaluation of alkyl triazoles and alkyl phosphocholines. Eur J Med Chem 2015; 101:24– 33.

Khamees AH, Mutlag SH, Al-Hilli FA, Bahjat AA. Evaluation of antibacterial activity of aqueous and methanol extract of Iraqi Althaea officinalis L. flowers on gastrointestinal key pathogens. Int. J. Pharm. Sci. Rev. Res. 2018;48(2):59-62.

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Published

2025-03-29