Preparation, In-vitro, and Ex-vivo Evaluation of Ondansetron Loaded Invasomes for Transdermal Delivery
DOI:
https://doi.org/10.31351/vol32iss3pp71-84Keywords:
Invasomes; Ondansetron; Transdermal; Lecithin; TerpenesAbstract
Invasomes are newly developed types of nanovesicles. A vesicular drug delivery system is considered one of the approaches for transdermal delivery to enhance permeation and improve drug bioavailability. Ondansetron is a serotonin receptor antagonist used for treating vomiting associated with different clinical cases. The study aimed to prepare invasomal dispersions for improving permeation of ondansetron across the skin with a controlled release pattern. Twenty-seven formulas of ondansetron-loaded invasomes were prepared by a modified mechanical dispersion method. These formulas were optimized by studying the effect of variables on entrapment efficiency. Vesicle size, polydispersity, zeta potential, in-vitro release and ex-vivo permeation studies were done for the optimized formulas. The selected formula was )F25( had )88.24%±0.04 (entrapment, (317.7 nm) vesicle size, (0.29) polydispersity, and (-31.5mV) zeta potential. In-vitro release study showed That (F25) had 75% release after (12) hrs., and dissolution followed the Korsmeyer-Peppas model with anomalous diffusion. Ex-vivo permeation study showed steady-state flux was 340.2 µg/cm2.hr with no lag time using rat skin tissue. A transmission electron microscope was done to visualize the selected formula. Invasomes are considered promising drug delivery systems for transdermal delivery of ondansetron, ensuring efficient permeation with a sustained release pattern.
How to Cite
Publication Dates
References
Hashmat D, Shoaib MH, Ali FR, Siddiqui F. Lornoxicam controlled release transdermal gel patch: Design, characterization, and optimization using co-solvents as penetration enhancers. PLoS ONE 2020;15(2):1–23. https://doi.org/10.1371/journal.pone.0228908
Priyanka K, Pentewar R, Bhusnure OG, Thonte SS, Supriya M, Sarda RR. Use of novel penetration enhancers and techniques in tdds. Indo American Journal of Pharmaceutical Research. 2015;5(9):2752–60. https://doi.org/10.1021/acs.molpharmaceut.3c00126
Jain S, Tripathi S, Tripathi PK. Invasomes: Potential vesicular systems for transdermal delivery of drug molecules. Journal of Drug Delivery Science and Technology. 2021; 61:102166. https:// doi.org/ 10.1016/ j.jddst.2020.102166
Tincello DG. Treatment of hyperemesis gravidarum with the 5-ht3 antagonist ondansetron (Zofran). Postgraduate Medical Journal. 1996;72(853):688–9. https://doi.org/10.1136/pgmj.72.853.688
Alotaibi BS, Pervaiz F, Buabeid M, Ashames A, Fahelelbom KM, Siddique S, et al. Nanostructured lipid carriers-based suppository for enhanced rectal absorption of ondansetron: In-vitro and in-vivo Evaluations. Arabian Journal of Chemistry. 2021;14(12): 103426. https://doi.org/10.1016/j.arabjc. 2021. 103426
Noor AH, Ghareeb MM. Formulation and evaluation of ondansetron hcl nanoparticles for transdermal delivery. Iraqi Journal of Pharmaceutical Sciences. 2020;29(2):70–9. https://doi.org/ 10.31351/vol29iss 2pp70-79
Ammar HO, Tadros MI, Salama NM, Ghoneim AM. Ethosome-derived invasomes as a potential transdermal delivery system for vardenafil hydrochloride: Development, optimization and application of physiologically based pharmacokinetic modeling in adults and geriatrics. International Journal of Nanomedicine. 2020; 15:5671–85. https://doi.org/10.2147/IJN.S261764
Habib BA, Sayed S, Elsayed GM. Enhanced transdermal delivery of ondansetron using nanovesicular systems: Fabrication, characterization, optimization and ex-vivo permeation study-box-cox transformation practical example. European Journal of Pharmaceutical Sciences. 2018; 115:352–61. https://doi.org/10.1016/j.ejps.2018.01.044
National committee for research ethics in science and techno. Guidelines for research ethics in science and technology. Jahrbuch für Wissenschaft und Ethik. 2009;14(1):255–66.
Kumar B, Sahoo PK, Manchanda S. Formulation, characterization and ex-vivo study of curcumin nano-invasomes gel for enhanced transdermal delivery. OpenNano. 2022; 7:100058. https://doi.org/10.1016/j. onano. 2022.100058
Veseli A, Žakelj S, Kristl A. A review of methods for solubility determination in biopharmaceutical drug characterization. Drug Development and Industrial Pharmacy. 2019;45(11):1717–24. https://doi.org/10. 1080/03639045.2019.1665062
Mura S, Manconi M, Sinico C, Valenti D, Fadda AM. Penetration enhancer-containing vesicles (pevs) as carriers for cutaneous delivery of minoxidil. International Journal of Pharmaceutics. 2009;380(1–2):72–9.https://doi.org/10.1016/j.ijpharm.2009.06.040
Vidya K, Lakshmi PK. Cytotoxic effect of transdermal invasomes anastrozole gel on mcf-7 breast cancer cell line. Journal of Applied Pharmaceutical Science. 2019;9(3):50–8.https://doi.org/10.7324/JAPS.20 19.90308
Albash R, Al-Mahallawi AM, Hassan M, Alaa-Eldin AA. Development and optimization of terpene-enriched vesicles (terpesomes) for effective ocular delivery of fenticonazole nitrate: In-vitro characterization and in-vivo assessment. International Journal of Nanomedicine. 2021; 16:609–21. https:// doi.org/10.2147/IJN.S274290
Alkawak RS, Rajab NA. Lornoxicam-loaded cubosomes: -preparation and in-vitro characterization. Iraqi Journal of Pharmaceutical Sciences.2022:17;31(1):144-53. https://doi.org/10.31351/vol31iss1pp144-153
Zhang Y, Huo M, Zhou J, Zou A, Li W, Yao C, et al. DDSolver: An add-in program for modeling and comparison of drug dissolution profiles. AAPS Journal 2010;12(3):263-71.https://doi.org/10.1208/ s12248-010-9185-1
Guillot AJ, Jornet-Mollá E, Landsberg N, Milián-Guimerá C, Montesinos MC, Garrigues TM, et al. Cyanocobalamin ultraflexible lipid vesicles: Characterization and in-vitro evaluation of drug-skin depth profiles. Pharmaceutics. 2021;13(3). https://doi.org /10.33 90/pharmaceutics13030418
Dutta Tejaswi DJK. Estimation of ondansetron hydrochloride by Rp-HPLC. EPRA International Journal of Research & Development (IJRD). 2020;5(5):29–34. https:// doi.org/10.36713/epra2016
Krishnaiah YSR, Rama B, Raghumurthy V, Ramanamurthy K V., Satyanarayana V. Effect of PEG6000 on the in-vitro and in-vivo transdermal permeation of ondansetron hydrochloride from EVA1802 membranes. Pharmaceutical Development and Technology. 2009;14(1):53–64. https://doi.org/10.1080/ 10837450802409404
Abd E, Yousef SA, Pastore MN, Telaprolu K, Mohammed YH, Namjoshi S, Grice JE, Roberts MS. Skin models for the testing of transdermal drugs. clinical pharmacology: Advances and applications 2016; 8:163. https://doi.org/10.2147/CPAA.S64788
Anitha P, Satyanarayana S V. Design and optimization of nano invasomes gel of glibenclamide and atenolol combination: In-vitro and in-vivo evaluation. Future Journal of Pharmaceutical Sciences 2021;7(1). https://doi.org/10.1186/s43094-021-00240-4
Badr-Eldin SM, Ahmed OAA. Optimized nano-transfersomal films for enhanced sildenafil citrate transdermal delivery: Ex-vivo and in-vivo evaluation. Drug Design, Development and Therapy 2016;10:1323–33. https://doi.org/10.2147/DDDT.S103122
Al Abood RM, Talegaonkar S, Tariq M, Ahmad FJ. Microemulsion as a tool for the transdermal delivery of ondansetron for the treatment of chemotherapy induced nausea and vomiting. Colloids and Surfaces B: Biointerfaces. 2013; 101:143–51. https:// doi.org/ 10.1016/j.colsurfb.2012.06.015
Abdulbaqi MR, Rajab NA. Preparation, characterization and ex-vivo permeability study of transdermal apixaban o/w nanoemulsion based gel. Iraqi Journal of Pharmaceutical Sciences. 2021;29(2):214–22. https://doi.org/10.31351/vol29iss2pp214-222
Thamer AK, Abood AN. Preparation and in-vitro characterization of aceclofenac nanosuspension (acns) for enhancement of percutaneous absorption using hydrogel dosage form. Iraqi Journal of Pharmaceutical Sciences. 2021;30(2):86-98. https://doi.org/10.31351/vol30iss2pp86-98
Sinko, Patrick J., et al. Physical chemical and biopharmaceutical principles in the pharmaceutical sciences. martin's physical pharmacy and pharmaceutical sciences.6th Ed., MD: Lippincott Williams &. Baltimore. 2011: 182-96
Rasheedy MM, El-mahdy MM, Fathallah D, Ibrahim EA. Bulletin of pharmaceutical formulation and evaluation of ondansetron transdermal gels. 2017; 40:57–70. https:// doi.org/10.21608/bfsa.2017.63166
Ahmed OAA, Badr-Eldin SM. Development of an optimized avanafil-loaded invasomes transdermal film: Ex-vivo skin permeation and in-vivo evaluation. International Journal of Pharmaceutics 2019; 570:118657. https://doi.org/10.1016/j. ijpharm.2019. 118657
Dsouza L, Chaudhari P, Brahmam B, Lewis SA. Derma roller mediated transdermal delivery of tizanidine invasomes for the management of skeletal muscle spasms. European Journal of Pharmaceutical Sciences. 2021; 165:105920. https:// doi. org/10.1016/j.ejps.2021.105920
El-Nabarawi MA, Shamma RN, Farouk F, Nasralla SM. Dapsone-loaded invasomes as a potential treatment of acne: Preparation, characterization, and in-vivo skin deposition assay. AAPS PharmSciTech 2018;19(5):2174–84. https://doi.org/10.1208/s12249-018-1025-0
Tabandeh H, Mortazavi SA. An investigation into some effective factors on encapsulation efficiency of alpha tocopherol in mlvs and the release profile from the corresponding liposomal gel. Iranian Journal of Pharmaceutical Research. 2013;12(SUPPL.):19–28.
Hammoud Z, Gharib R, Fourmentin S, Elaissari A, Greige-Gerges H. New findings on the incorporation of essential oil components into liposomes composed of lipoid s100 and cholesterol. International Journal of Pharmaceutics. 2019; 561:161–70. https:// doi.org/10.1016/j.ijpharm.2019.02.022
Midekessa G, Godakumara K, Ord J, Viil J, Lättekivi F, Dissanayake K, et al. Zeta potential of extracellular vesicles: toward understanding the attributes that determine colloidal stability. ACS Omega. 2020;5(27):16701–10. https://doi.org/10.1021/ acsomega. 0c01582
Sinico C, Manconi M, Peppi M, Lai F, Valenti D, Fadda AM. Liposomes as carriers for dermal delivery of tretinoin: in-vitro evaluation of drug permeation and vesicles–skin interaction. Journal of Controlled Release. 2005;103(1):123-36. https://doi. org/10.1016/j.jconrel.2004.11.020
Katahira N, Murakami T, Kugai S, Yata N, Takano M. Enhancement of topical delivery of a lipophilic drug from charged multilamellar liposomes. Journal of Drug Targeting. 1999;6(6):405-14. https:// doi.org/10.3109/10611869908996847
Sinico C, Valenti D, Manconi M, Lai F, Fadda AM. Cutaneous delivery of 8-methoxypsoralen from liposomal and niosomal carriers. Journal of Drug Delivery Science and Technology. 2006;16(2):115-20. https://doi.org/10.1016/S1773-2247 (06)50017-6
Wu IY, Bala S, Škalko-Basnet N, di Cagno MP. Interpreting non-linear drug diffusion data:utilising korsmeyer-peppas model to study drug release from liposomes. European Journal of Pharmaceutical Sciences. 2019; 138:105026. https://doi.org/10.1016/j. ejps.2019.105026
Choube MKD and RN. Formulation and evaluation of transdermal therapeutic system of ondansetron hydrochloride. Current Trends in Pharmaceutical Research. 2012;1(1):13–32.
Narishetty ST, Panchagnula R. Transdermal delivery of zidovudine: effect of terpenes and their mechanism of action. Journal of Controlled Release. 2004;95(3):367-79. https://10.1016/j.jconrel.2003 .11.022
El-Kattan AF, Asbill CS, Kim N, Michniak BB. The effects of terpene enhancers on the percutaneous permeation of drugs with different lipophilicities. International Journal of Pharmaceutics. 2001;215(1–2):229–40. https://doi.org/10.1016/s0378-5173(00)00699-2
Ahad A, Al-Mohizea AM, Al-Jenoobi FI, Aqil M. Transdermal delivery of angiotensin ii receptor blockers (arbs), angiotensin-converting enzyme inhibitors (aceis) and others for management of hypertension. Drug Delivery. 2016;23(2):579–90. https://doi.org/10.3109/10717544.2014.942444
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)
This work is licensed under a Creative Commons Attribution 4.0 International License.
