Association of Apelin, Chemerin and Omentin Levels with Oxidative Stress Markers in Non-Diabetic and Induced Diabetic Rats
DOI:
https://doi.org/10.31351/vol33iss1pp104-112Keywords:
Keywords: Induce type one diabetes mellitus, Apelin, Chemerin, OmentinAbstract
Abstract
Oxidative stress has a significant impact on the etiology of type 1 diabetes mellitus (T1DM). However, associations of adipokines with oxidative stress biomarkers have yet to be investigated. Therefore, the present study aimed to investigate the estimation of serum apelin, chemerin, and omentin levels in induced TIDM and their correlations with oxidative stress markers. The study included sixty male albino rats divided into two groups. The first group (n = 30) was diabetic (TIDM induced with an intraperitoneal injection of 40mg/kg STZ). The non-diabetic control group was the second (n: 30). The enzyme-linked immunosorbent (ELISA) test was used to quantify the serum levels of apelin, chemerin, omentin and asymmetric dimethylarginine (ADMA). Furthermore, blood glucose, lipid profile triglyceride (TG), cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), liver enzymes (alanine aminotransferase (ALT) and aspartate transaminase (AST)), malondialdehyde (MDA) and nitric oxide (NO) parameters were evaluated. Rats with induced TIDM had significantly higher serum levels of apelin, chemerin, omentin, malondialdehyde MDA, ADMA, and NO compared to non-diabetics. According to the coefficient of correlation analysis, the results revealed a positive association between apelin and LDL, ALT, and MDA and a negative correlation between apelin and AST: ALT. In addition, no correlation was found between apelin and serum levels of glucose, cholesterol, HDL, LDL: HDL TG, TG: HDL, VLDL, AST, creatinine, uric acid, albumin, NO, and ADMA. LDL, ALT, and MDA. Serum chemerin level correlated positively with LD: HDL and ADMA. Also, the plasma level of omentin showed a significant and positive correlation with both TG: HDL and MDA values. In conclusion, the results of this study introduced apelin and chemerin as potential biomarkers for the early detection of diabetes. Elevated serum apelin levels in induced T1DM could provide compensatory action for low insulin levels. While, apelin, chemerin and omentin’s positive correlations with MDA level suggest the potential roles of these adipokines in diabetes-induced complications caused by oxidative stress.
Received: 27/11 /2022
Accepted: 5/4 /2023
References
As Habi A, Sadeghi M, Arab A, Hajianfar H. The association between omentin and diabetes: a systematic review and meta-analysis of observational studies. Diabetes Metab Syndr Obes. 2019;12:1277-86.
Pasupuleti VR, Arigela CS, Gan SH, Salam SKN, Krishnan KT, Rahman NA, et al. A Review on Oxidative Stress, Diabetic Complications, and the Roles of Honey Polyphenols. Oxid Med Cell Longev. 2020;2020:8878172.
Gawrys J, Gajecki D, Szahidewicz-Krupska E, Doroszko A. Intraplatelet L-Arginine-Nitric Oxide Metabolic Pathway: From Discovery to Clinical Implications in Prevention and Treatment of Cardiovascular Disorders. Oxid Med Cell Longev. 2020;2020:1015908.
Lv S, Feng Y, Jiang Q, Lv X, Yang Y, Cirillo G. Relationship between Apelin/APJ Signaling, Oxidative Stress, and Diseases. Oxidative Medicine and Cellular Longevity. 2021;2021:1-7.
Zhou Q, Cao J, Chen L. Apelin/APJ system: A novel therapeutic target for oxidative stress-related inflammatory diseases (Review). Int J Mol Med. 2016;37(5):1159-69.
Lu T, Lee HC. Coronary Large Conductance Ca(2+)-Activated K(+) Channel Dysfunction in Diabetes Mellitus. Front Physiol. 2021;12:750618.
Helfer G, Wu QF. Chemerin: a multifaceted adipokine involved in metabolic disorders. J Endocrinol. 2018;238(2):R79-r94.
Neves KB, Nguyen Dinh Cat A, Alves-Lopes R, Harvey KY, Costa RMD, Lobato NS, et al. Chemerin receptor blockade improves vascular function in diabetic obese mice via redox-sensitive and Akt-dependent pathways. Am J Physiol Heart Circ Physiol. 2018;315(6):H1851-H60.
Zhou Y, Zhang B, Hao C, Huang X, Li X, Huang Y, et al. Omentin-A Novel Adipokine in Respiratory Diseases. Int J Mol Sci. 2017;19(1).
Liu F, Fang S, Liu X, Li J, Wang X, Cui J, et al. Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway. Biochem Pharmacol. 2020;174:113830.
Eimal Latif AH, Anwar S, Gautham KS, Kadurei F, Ojo RO, Hafizyar F, et al. Association of Plasma Omentin-1 Levels With Diabetes and Its Complications. Cureus. 2021;13(9):e18203.
Abdelraouf Korany M, Sonbol A, Mohamed Elgouhary S. Omentin-1 and diabetic retinopathy in type 2 diabetic patients. Alexandria journal of medicine. 2018;54(4):323-6.
Hayashi M, Morioka T, Hatamori M, Kakutani Y, Yamazaki Y, Kurajoh M, et al. Plasma omentin levels are associated with vascular endothelial function in patients with type 2 diabetes at elevated cardiovascular risk. Diabetes Research and Clinical Practice. 2019;148:160-8.
Yanni AE, Antoniou N, Kostomitsopoulos N. Improvements needed in reporting the methodology for STZ-induced diabetes in rats. Am J Physiol Endocrinol Metab. 2021;320(5):E898-E9.
Ratajczak-Wrona W, Jablonska E, Antonowicz B, Dziemianczyk D, Grabowska SZ. Levels of biological markers of nitric oxide in serum of patients with squamous cell carcinoma of the oral cavity. International Journal of Oral Science. 2013;5(3):141-5.
Ali IMM, Yenzeel JH, Al-ansari HMS. Evaluation of Oxidative Stress and LeptinLevel in Samples of Iraqi Obese Women. Iraqi Journal of Science. 2020:1565-70.
Habchi M, Duvillard L, Cottet V, Brindisi MC, Bouillet B, Beacco M, et al. Circulating apelin is increased in patients with type 1 or type 2 diabetes and is associated with better glycaemic control. Clin Endocrinol (Oxf). 2014;81(5):696-701.
Li M, Fang H, Hu J. Apelin‑13 ameliorates metabolic and cardiovascular disorders in a rat model of type 2 diabetes with a high‑fat diet. Mol Med Rep. 2018;18(6):5784-90.
Li C, Cheng H, Adhikari BK, Wang S, Yang N, Liu W, et al. The Role of Apelin-APJ System in Diabetes and Obesity. Front Endocrinol (Lausanne). 2022;13:820002.
Huang Q, Liu X, Cao C, Lei J, Han D, Chen G, et al. Apelin-13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway-dependent upregulation of Beclin1. Oncol Lett. 2016;11(2):1051-6.
Shahvali S, Shahesmaeili A, Sanjari M, Karami-Mohajeri S. The correlation between blood oxidative stress and sialic acid content in diabetic patients with nephropathy, hypertension, and hyperlipidemia. Diabetol Int. 2020;11(1):19-26.
Yamazaki S, Sekiguchi A, Uchiyama A, Fujiwara C, Inoue Y, Yokoyama Y, et al. Apelin/APJ signaling suppresses the pressure ulcer formation in cutaneous ischemia-reperfusion injury mouse model. Sci Rep. 2020;10(1):1349.
Bi Y, Lei X, Chai N, Linghu E. NOX4: a potential therapeutic target for pancreatic cancer and its mechanism. Journal of translational medicine. 2021;19(1):1-8.
Liu M, Li H, Zhou Q, Zhao H, Lv D, Cao J, et al. ROS-Autophagy pathway mediates monocytes-human umbilical vein endothelial cells adhesion induced by apelin-13. J Cell Physiol. 2018;233(10):6839-50.
Chen L, Zhou T, White T, O'Brien A, Chakraborty S, Liangpunsakul S, et al. The Apelin-Apelin Receptor Axis Triggers Cholangiocyte Proliferation and Liver Fibrosis During Mouse Models of Cholestasis. Hepatology. 2021;73(6):2411-28.
Sokolovska J, Dekante A, Baumane L, Pahirko L, Valeinis J, Dislere K, et al. Nitric oxide metabolism is impaired by type 1 diabetes and diabetic nephropathy. Biomed Rep. 2020;12(5):251-8.
Meza CA, La Favor JD, Kim DH, Hickner RC. Endothelial Dysfunction: Is There a Hyperglycemia-Induced Imbalance of NOX and NOS? Int J Mol Sci. 2019;20(15).
Oxidative Stress and Endothelial Dysfunction in Sepsis and Acute Inflammation. Antioxidants & Redox Signaling. 2021;35(15):1291-307.
Wang Y, Song J, Bian H, Bo J, Lv S, Pan W, et al. Apelin promotes hepatic fibrosis through ERK signaling in LX-2 cells. Mol Cell Biochem. 2019;460(1-2):205-15.
Arroyave-Ospina JC, Wu Z, Geng Y, Moshage H. Role of Oxidative Stress in the Pathogenesis of Non-Alcoholic Fatty Liver Disease: Implications for Prevention and Therapy. Antioxidants. 2021;10(2):174.
Sun J, Ren J, Zuo C, Deng D, Pan F, Chen R, et al. Circulating apelin, chemerin and omentin levels in patients with gestational diabetes mellitus: a systematic review and meta-analysis. Lipids Health Dis. 2020;19(1):26.
Elsehmawy A, El-Toukhy SE, Seliem NMA, Moustafa RS, Mohammed DS. Apelin and chemerin as promising adipokines in children with type 1 diabetes mellitus. Diabetes Metab Syndr Obes. 2019;12:383-9.
Akgul Balaban Y, Yilmaz N, Kalayci M, Unal M, Turhan T. IRISIN AND CHEMERIN LEVELS IN PATIENTS WITH TYPE 2 DIABETES MELLITUS. Acta Endocrinol (Buchar). 2019;15(4):442-6.
Lőrincz H, Katkó M, Harangi M, Somodi S, Gaál K, Fülöp P, et al. Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects. Clin Endocrinol (Oxf). 2014;81(3):370-7.
Szpakowicz A, Szpakowicz M, Lapinska M, Paniczko M, Lawicki S, Raczkowski A, et al. Serum Chemerin Concentration Is Associated with Proinflammatory Status in Chronic Coronary Syndrome. Biomolecules. 2021;11(8).
Spiroglou SG, Kostopoulos CG, Varakis JN, Papadaki HH. Adipokines in periaortic and epicardial adipose tissue: differential expression and relation to atherosclerosis. J Atheroscler Thromb. 2010;17(2):115-30.
Gac P, Poreba M, Jurdziak M, Trzmielewska E, Goclawska K, Derkacz A, et al. Cardiovascular risk factors and the concentration of asymmetric dimethylarginine. Adv Clin Exp Med. 2020;29(1):63-70.
Tan BK, Adya R, Farhatullah S, Chen J, Lehnert H, Randeva HS. Metformin treatment may increase omentin-1 levels in women with polycystic ovary syndrome. Diabetes. 2010;59(12):3023-31.
Watanabe T, Watanabe-Kominato K, Takahashi Y, Kojima M, Watanabe R. Adipose Tissue-Derived Omentin-1 Function and Regulation. Compr Physiol. 2017;7(3):765-81.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Iraqi Journal of Pharmaceutical Sciences( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)
This work is licensed under a Creative Commons Attribution 4.0 International License.
