Possible Effects of Vitamin D3 and Levofloxacin on Selected Hematology Parameter of Rats

Authors

  • Abbas Muslim Mhaibes Ministry of Health and Environment, Missan Health Directorate, Missan, Iraq.
  • Farah Kais Abdul- Wahab2 Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Iraq.

DOI:

https://doi.org/10.31351/vol32issSuppl.pp74-84

Keywords:

Levofloxacin , vitaminD3 , Haematology ,anaemia , rats.

Abstract

Vitamin D is a fat-soluble vitamin with antioxidant and DNA protecting properties , Levofloxacin is a member of the fluoroquinolone drug class, Its broad-spectrum bactericidal effect affects both Gram-positive and Gram-negative bacteria.

The goal of the study is to analyze the haematology analysis in rats received levofloxacin and show the preventive impact of vitamin D3 by analyzing the haematology parameters: packed cell volume (PCV), mean corpuscular hemoglobin concentration(MCHC),haemoglobin (HB), red blood cell (RBC), mean corpuscular volume (MCV),meancorpuscular haemoglobin(MCH), WBC ,differential WBC, and Platelets.

The study included 42 rats divided into 6 groups each group 7 rats. group I negative control : healthy animals received normal saline 0.9%, group II: received levofloxacin LFX dose of 50mg/kg/day (IP) for fourteen days, group III : received LFX 100mg/kg/day (IP) for fourteen days, group IV : received vitamin D3 500 IU/day orally by oral gavage for twenty one days, group V received vitamin D3 500 IU/day orally for twenty one days and levofloxacin 50 mg/kg/day IP injected at day 8 for fourteen days, group VI received vitaminD3 500IU/day for twenty one days orally and levofloxacin 100 mg/kg/day IP injected at day 8 for fourteen days.

  Blood samples taken from rats treated with levofloxacin, showed a decrease in the values of RBC, HB, PCV, MCH, MCHC, as well as a decrease in total white blood cells, then returned approximately to normal levels in the groups IV ,group V and group VI .

from the result of the study conclude that some haematological  changes caused by levofloxacin ameliorated by vitamin D3 may be due to indirect effect of vitamin D3 on haematology parameters.

 

How to Cite

1.
Mhaibes AM, Abdul- Wahab2 FK. Possible Effects of Vitamin D3 and Levofloxacin on Selected Hematology Parameter of Rats . Iraqi Journal of Pharmaceutical Sciences [Internet]. 2023 Nov. 1 [cited 2024 Dec. 28];32(Suppl.):74-8. Available from: https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/2516

Publication Dates

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Published

2023-11-01