A Comparative Biochemical Study of Proteins Profile in Iraqi Children and Adolescent with ?-Thalassemia
The aim of the present research is to study different protein fractions in sera of children and adolescent with Î² â€“thalassemia major and minor and to compare the results with that of healthy control.One hundred fifty children and adolescents were enrolled in this study,including 50 patients with Î²- thalassemia major , 50 patients with Î²- thalassemia minor as pathological control group and another apparently 50 healthy individuals as a control group. The age of all studied groups ranged from (4-18)years.Total protein, albumin and immunoglobulins were estimated in sera of all subjects. A Significant decrease was found in the total protein and albumin levels in sera of both major and minor thalassemic patients compared to normal groups. A Significant increase in immunoglobulin levels (IgG, IgM and IgA) was found in the sera of major and minor Î²-thalassemia patients compared to control.Different protein parts in sera of all subjects were detected using cellulose acetate electrophoresis.The results revealed significant reduction in Î²- globulin fractions in Î²- thalassemia major patients compared to the normal and pathological control groups. Significant elevations in Î³- globulins fractions were observed in both major Î²- thalassemia and minor Î²- thalassemia as compared to normal control group. As a Conclusion the alteration in some protein parts occurred which is more obvious in major thalassemia patients compared to the normal and pathological control groups.
Key words: Protein Parts , Elecrtrophoresis , Î²-Thalassemia