Quantitative Determination and Cytotoxic Effect of Oleanolic Acid from Olea europaea Leaves Extract Cultivated in Iraq
DOI:
https://doi.org/10.31351/vol31iss2pp244-250Keywords:
Oleanolic acid, acidic hydrolysis method, basic acidic method, HPLC, AMJ13 cell line.Abstract
Since its first description as a cytotoxic agent, Olea europaea leaves extract gained significant popularity against human breast cancer, ethyl acetate extract of Olea europaea leaves obtained by acid hydrolysis method was evaluated in vitro as cytotoxic agent against new human breast cancer (AMJ13) cell line, using the MTT assay. One main pentacyclic triterpenoid; oleanolic acid, was isolated from leaves of Olea europaea by well-known two different methods, but not used for this compound before, the acidic hydrolysis method and basic acidic method. The presence of oleanolic acid was proved in both methods with qualitative and quantitative determination using high performance liquid chromatography (HPLC), which was active against breast cancer AMJ13 cell line with IC50 value of 0.8936 ?g/ml.
References
Assumaidaee AAM, Ali NM, Ibraheem ZO, Fadhil AA, AL-HELLI KAA. Anti-Oxidoreductive stress Effects of Iraqi Olea Europaea L. Leaves Extract against Low Double Doses of Alloxan Induced Diabetes Mellitus in Rats. Systematic Reviews in Pharmacy. 2020;11(1):292-302.
Kabbash EM, Ayoub IM, Abdel-Shakour ZT, El-Ahmady SH. A phytochemical study on Olea europaea L. Olive leaf extract (cv. Koroneiki) growing in Egypt. Archives of Pharmaceutical Sciences Ain Shams University. 2019;3(1):99-105.
Masoko P, Makgapeetja DM. Antibacterial, antifungal and antioxidant activity of Olea africana against pathogenic yeast and nosocomial pathogens. BMC complementary and alternative medicine. 2015;15(1):1-9.
Nicolì F, Negro C, Vergine M, Aprile A, Nutricati E, Sabella E, et al. Evaluation of phytochemical and antioxidant properties of 15 Italian Olea europaea L. cultivar leaves. Molecules. 2019;24(10):1998.
Guinda A, Rada M, Delgado T, Gutierrez-Adanez P, Castellano JMa. Pentacyclic triterpenoids from olive fruit and leaf. Journal of agricultural and food chemistry. 2010;58(17):9685-91.
Luchnikova NA, Grishko VV, Ivshina IB. Biotransformation of oleanane and ursane triterpenic acids. Molecules. 2020;25(23):5526.
Naumoska K, Simonovska B, Albreht A, Vovk I. TLC and TLC-MS screening of ursolic, oleanolic and betulinic acids in plant extracts. JPC-Journal of Planar Chromatography-Modern TLC. 2013;26(2):125-31.
Ayeleso TB, Matumba MG, Mukwevho E. Oleanolic acid and its derivatives: biological activities and therapeutic potential in chronic diseases. Molecules. 2017;22(11):1915.
Pollier J, Goossens A. Oleanolic acid. Phytochemistry. 2012;77:10-5.
Córdova C, Gutiérrez B, Martínez-García C, Martín R, Gallego-Muñoz P, Hernández M, et al. Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis. PLoS One. 2014;9(4):e91282.
Gudoityte E, Arandarcikaite O, Mazeikiene I, Bendokas V, Liobikas J. Ursolic and Oleanolic Acids: Plant Metabolites with Neuroprotective Potential. International Journal of Molecular Sciences. 2021;22(9):4599.
He H, Li H, Akanji T, Niu S, Luo Z, Li D, et al. Synthesis and biological evaluations of oleanolic acid indole derivatives as hyaluronidase inhibitors with enhanced skin permeability. Journal of Enzyme Inhibition and Medicinal Chemistry. 2021;36(1):1665-78.
Shi Y, Song Q, Hu D, Zhuang X, Yu S, Teng D. Oleanolic acid induced autophagic cell death in hepatocellular carcinoma cells via PI3K/Akt/mTOR and ROS-dependent pathway. The Korean Journal of Physiology & Pharmacology. 2016;20(3):237-43.
Wang H, Zhong W, Zhao J, Zhang H, Zhang Q, Liang Y, et al. Oleanolic acid inhibits epithelial–mesenchymal transition of hepatocellular carcinoma by promoting iNOS dimerization. Molecular cancer therapeutics. 2019;18(1):62-74.
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Copyright (c) 2022 Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512)
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