The Potential Hepatoprotective Effect of Vinpocetine against Lead-Induced Inflammatory and Apoptotic Cytokines in Rats
DOI:
https://doi.org/10.31351/vol33iss3pp56-62Keywords:
Keyword: Hepatoprotective, Inflammation,Apoptotic,Lead, Vinpocetine.Abstract
Abstract
Environmental pollution with heavy metals like lead has become a matter of concern, the accumulation and multi-systemic toxicity of lead affect most body organs and its toxicity is related with many pathological changes, especially on liver. 18 Sprague-Dawley rats weighting 160-250g of both male and female were included; the animals were divided with randomness into three groups, 6 rats each group: 1st group: Rats were orally inoculated with 0.3 ml saline, after 1 hour, intraperitoneal (IP) injection of 100 μl of saline was given (Control). 2nd group: Rats received daily IP injection (20 mg/kg body wt.) of immediately preparation lead acetate for 5 days, the dose and route of administration were chose based on previous research. 3rd group Rats in this group received vinpocetine and lead as the following; at first vinpocetine administered orally by gavage tube in a dose of 3mg / kg every day for five days alone and then lead injection started in a dose of 20 mg/kg and continued for 10 days with oral vinpocetine dose where vinpocetine administered 1hr before lead. After 24 hrs (end of management period), each rat was anesthetized by diethyl ether.
Liver homogenates were done; then interleukin-1 beta (IL-1β), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α) and caspase 3 were estimated.
Lead was significantly (P < 0.05) raised IL-1beta, TNF-α and caspase 3, whereasit significantly (P < 0.05) reducedIL-10 levels. On the other hand, vinpocetine significantly (P < 0.05) lowered IL-1beta, caspase 3 and it significantly (P < 0.05) raised IL-10 but it didn’t significantly (P > 0.05) reduce TNF-α.
Vinpocetine may have potential hepatoprotective effect against lead-induced toxicity.
Keywords: Apoptotic, Hepatoprotective, Inflammation, Lead, Vinpocetine.
Received 12/5/2023
Accepted 28/8/2023
Published 15/9/2024
References
Haouas Z, Sallem A, Zidi I, Hichri H, Mzali I, Mehdi M. Hepatotoxic effects of lead acetate in rats: histopathological and cytotoxic studies. Journal of Cytology & Histology. 2014;5(5):1.
Kumar MR, Reddy KS, Reddy AG, Reddy RA, Anjaneyulu Y, Reddy DG. Lead-induced Hepatotoxicity and Evaluation of Certain Anti-stress Adaptogens in Poultry. Toxicology international. 2011;18(1):62-6.
Maret W. The Bioinorganic Chemistry of Lead in the Context of Its Toxicity. Metal ions in life sciences. 2017;17.
Grandjean P. Widening perspectives of lead toxicity. A review of health effects of lead exposure in adults. Environmental research. 1978;17(2):303-21.
Al-Megrin WA, Alkhuriji AF, Yousef AOS, Metwally DM, Habotta OA, Kassab RB, et al. Antagonistic Efficacy of Luteolin against Lead Acetate Exposure-Associated with Hepatotoxicity is Mediated via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Activities. Antioxidants (Basel, Switzerland). 2019;9(1).
Mudipalli A. Lead hepatotoxicity & potential health effects. The Indian journal of medical research. 2007;126(6):518-27.
Hegazy AMS, Fouad UA. Evaluation of Lead Hepatotoxicity; Histological, Histochemical and Ultrastructural Study. Forensic Medicine and Anatomy Research. 2014; Vol. 02 No .03 :10.
Sirivarasai J, Wananukul W, Kaojarern S, Chanprasertyothin S, Thongmung N, Ratanachaiwong W, et al. Association between inflammatory marker, environmental lead exposure, and glutathione S-transferase gene. BioMed research international. 2013;2013:474963.
Alhusaini A, Fadda L, Hasan IH, Zakaria E, Alenazi AM, Mahmoud AM. Curcumin Ameliorates Lead-Induced Hepatotoxicity by Suppressing Oxidative Stress and Inflammation, and Modulating Akt/GSK-3β Signaling Pathway. Biomolecules. 2019;9(11).
Khan DA, Qayyum S, Saleem S, Khan FA. Lead-induced oxidative stress adversely affects health of the occupational workers. Toxicology and industrial health. 2008;24(9):611-8.
Sharma A, Sharma V, Kansal L. Amelioration of lead-induced hepatotoxicity by Allium sativum extracts in Swiss albino mice. The Libyan journal of medicine. 2010;5(1):4621.
Abdel Salam OM, Oraby FH, Hassan NS. Vinpocetine ameliorates acute hepatic damage caused by administration of carbon tetrachloride in rats. Acta biologica Hungarica. 2007;58(4):411-9.
lnfarawyhttps A, Nashy A, Abozaid A , et al.Vinpocetine attenuates thioacetamide-induced liver fibrosis in rats. Human & Experimental Toxicology.2021;40(2)Pages 355-368
EZaki HF, Abdelsalam RM. Vinpocetine protects liver against ischemia-reperfusion injury. Canadian journal of physiology and pharmacology. 2013;91(12):1064-70.
Billakota S, Andresen JM, Gay BC, Stewart GR, Fedorov NB, Gerlach AC, et al. Personalized medicine: Vinpocetine to reverse effects of GABRB3 mutation. Epilepsia. 2019;60(12):2459-65.
Wu MP, Zhang YS, Xu X, Zhou Q, Li JD, Yan C. Vinpocetine Attenuates Pathological Cardiac Remodeling by Inhibiting Cardiac Hypertrophy and Fibrosis. Cardiovascular drugs and therapy. 2017;31(2):157-66.
Abdel Moneim AE. Indigofera oblongifolia Prevents Lead Acetate-Induced Hepatotoxicity, Oxidative Stress, Fibrosis and Apoptosis in Rats. PloS one. 2016;11(7):e0158965.
Nadeem RI, Ahmed HI, El-Sayeh BM. Protective effect of vinpocetine against neurotoxicity of manganese in adult male rats. Naunyn-Schmiedeberg's archives of pharmacology. 2018;391(7):729-42.
Ruiz-Miyazawa KW, Zarpelon AC, Pinho-Ribeiro FA, Pavão-de-Souza GF, Casagrande R, Verri WA, Jr. Vinpocetine reduces carrageenan-induced inflammatory hyperalgesia in mice by inhibiting oxidative stress, cytokine production and NF-κB activation in the paw and spinal cord. PloS one. 2015;10(3):e0118942.
Fattori V, Borghi SM, Guazelli CFS, Giroldo AC, Crespigio J, Bussmann AJC, et al. Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-κB activation in mice. Pharmacological research. 2017;120:10-22.
Abd allah ESH, Badary DM. Folic acid protects against lead acetate-induced hepatotoxicity by decreasing NF-κB, IL-1β production and lipid peroxidation mediataed cell injury. Pathophysiology. 2017;24(1):39-44.
Soliman MM, Baiomy AA, Yassin MH. Molecular and Histopathological Study on the Ameliorative Effects of Curcumin Against Lead Acetate-Induced Hepatotoxicity and Nephrototoxicity in Wistar Rats. Biological trace element research. 2015;167(1):91-102.
Turner MD, Nedjai B, Hurst T, Pennington DJ. Cytokines and chemokines: at the crossroads of cell signalling and inflammatory disease. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 2014;1843(11):2563-82.
Choudhary GS, Al-Harbi S, Almasan A. Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis. Methods in molecular biology (Clifton, NJ). 2015;1219:1-9.
Lin L, Yuan F, Liu Y, Zhong M, Xie T, Ni J, et al. Hepatotoxicity and mechanism study of chrysophanol-8-O-glucoside in vitro. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2019;120:109531.
Agarwal S, Roy S, Ray A, Mazumder S, Bhattacharya S: Arsenic trioxide and lead acetate induce apoptosis in adult rat hepatic stem cells. Cell Biol Toxicol. (2009), 25(4), 403-13.
Yedjou CG, Milner JN, Howard CB, Tchounwou PB: Basic apoptotic mechanisms of lead toxicity in human leukemia (HL-60) cells. Int J Environ Res Public Health (2010), 7(5), 2008-17
Soussi A, Gargouri M, El Feki A. Potential immunomodulatory and antioxidant effects of walnut Juglans regia vegetable oil against lead-mediated hepatic damage and their interaction with lipase activity in rats. Environmental toxicology. 2018;33(12):1261-71.
Almasmoum H, Refaat B, Ghaith MM, Almaimani RA, Idris S, Ahmad J, et al. Protective effect of Vitamin D3 against lead induced hepatotoxicity, oxidative stress, immunosuppressive and calcium homeostasis disorders in rat. Environmental toxicology and pharmacology. 2019;72:103246.
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