Evaluation of the Protective Effect of Echinacea purpurea on Iron Overload-Induced Multi-Organ Damage in Male Rats: In Comparison with Vitamin E
DOI:
https://doi.org/10.31351/vol35iss1pp179-189Abstract
By producing reactive oxygen species, primarily reactive hydroxyl radicals, iron overload may contribute to cellular damage and change the integrity of essential organelles. Echinacea Purpura; a medical plant with a root liquid extract contains various biologically active phytochemical compounds. This study aimed to evaluate the protective effect of Echinacea Purpura on bone marrow suppression with liver and spleen cells apoptosis induced by iron overload. Fifty albino rats were divided into five groups: Group (I) [negative control] intraperitoneally injection with (0.2 ml) normal saline solution every 3 days; with daily oral gavage of (1 ml) normal saline for 4 weeks. Group (II) [Iron-overloaded]: Rats intraperitoneally injected with (200 mg/kg/day iron dextran every 3 days); with daily oral gavage of (1 ml) normal saline solution for 4 weeks. Group (III) Rats intraperitoneally injected with iron dextran (200 mg/kg/day every 3 days)+Echinacea (100mg/kg/day) orally administered for 4 weeks. Group (IV) Rats intraperitoneally injected with iron dextran (200 mg/kg/day every 3 days)+Echinacea (200mg/kg/day) orally administered for 4 weeks. Groups (V) Rats intraperitoneally injected with iron dextran (200 mg/kg/day every 3 days) and an oil solution of vitamin E (200mg/kg/day) orally administered for 4 weeks. Twenty-four hours following the treatment duration; the animals were euthanized, sacrificed and blood was drawn from the carotid artery in the neck of each male rat to be utilized for the estimation of serum Ferritin, and Hepcidin; liver tissue homogenate for malondialdehyde, superoxide dismutase2, and glutathione biomarkers estimation and liver histopathological examination; spleen tissue homogenate utilized to estimate Nuclear factor erythroid 2- related factor 2 and Caspase-3 protein expression. Echinacea Purpura in 200mg showed significant elevation (p<0.05) in the serum hepcidin and antioxidant superoxide dismutase2, glutathione, and Nuclear factor erythroid 2- related factor 2 protein expression levels; with a significant reduction in Ferritin and Malondialdehyde levels and a non-significant elevation in Caspase-3 level.
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Lijia Zhang, Xuezheng Dai, Li Wang, Jingming Cai, Jie Shen, Yang Shen, Xianan Li and Yan Zhao, Iron overload accelerated lipid metabolism disorder and liver injury in rats with non-alcoholic fatty liver disease, Frontiers in Nutrition, 2022, 9: 961-892.
Zwe-Ling Kong, Athira Johnson, Tzu-Ling Ting, Po-Jen Cheng, and Chien-Feng Mao, Protective Effects of Echinacea purpurea Ethanol Extract on Male Reproductive Dysfunction in Obese Rats, Applied science, 2021, 11: 23-92.
Shihab Hattab Mutlag, Sajida Hussien Ismael, Ali Faris Hassan, Ruaa Fadhil Abbas, Genotoxic Effect of Ethyl acetate Fraction of Cressa cretica on Chromosomal Aberration on Bone Marrow Cells and Spleen Cells in Mice, International Journal of Pharmaceutical Sciences Review and Research, 2017, 39: 220-223.
Alaa Radhi Khudhair, Nada N. Al-Shawi and Ali Faris Hassan, Impact of Omega 3 on the Genotoxicity of Irinotecan on Bone Marrow and Spleen of Rats: in-vivo Study, Iraqi Journal of Pharmaceutical Science, 2023, 32(1): 53-58.
Scaramellini, N. et al. Interpreting iron homeostasis in congenital and acquired disorders, Pharmaceuticals, 2023, 16: 329.
ShimaaA. Sadek, Mohamed Marzouk, Hanan R. H. Mohamed, Bassant F.Abd El sallam, AbdoA. Elfky & AmanyA. Sayed, Chia seeds and coenzyme Q10 alleviate iron overload induced hepatorenal toxicity in mice via iron chelation and oxidative stress modulation, Scientific Reports, 2023, 13:19773.
Hala A.H. Khattab, Seham K. Abounasef, Haneen L. Bakheet, The Biological and Hematological Effects of Echinacea purpurea L. Roots Extract in the Immunocompromised Rats with Cyclosporine, Journal of Microscopy and Ultrastructure, 2019, 7, 2, 65-71.
Monique Aucoin, Valentina Cardozo, Meagan D.McLaren, Anna Garber, Daniella Remy, Joy Baker, Adam Gratton, Mohammed Ali Kala, Sasha Monteiro, Cara Warder, Alessandra Perciballi, Kieran Cooley, A systematic review on the effects of Echinacea supplementation on cytokine levels, Metabolism Open, 2021, 10, (1016): 1-32.
Emad Mohamed EL-SHERBINY, Hala Fawzy OSMAN, Mervat Sayed TAHA, Effectiveness of Echinacea purpurea Extract On Immune Deficiency Induced by Azathioprine In Male Albino Rats, Bioscience Journal, 2021, 37: 37029, 1981-3163.
Noor S Jaafar, Ali F Hassan and Maha N Hamad, Evaluation of the Genotoxicity of the Aerial Parts of Iraqi Euphorbia cyathophora on Bone Marrow and Spleen Cells in Mice, Iraqi Journal of Pharmaceutical Science, Genotoxic evaluation of euphorbia cyathophora, 2023, 32: 41-45.
Ahmed Hamed Jwaid, Ali Faris Hassan, Ali Abdulhussain Kasim, The Effect of Artemisia Dracunculus L. on Mitotic Index in Bone Marrow and Spleen Cells of Mice: In Vivo Study, Indian Journal of Forensic Medicine & Toxicology,2021, 15: No. 2 1879.
Manayi, A.; Vazirian, M.; Saeidnia, S. Echinacea purpurea: Pharmacology, phytochemistry and analysis methods. Pharmacogn. Rev. 2015, 9: 63–72.
Amani Jabber, Nada N. Al-Shawi, Ali F. Hasan, Evaluation of the Possible Protective Effect of Fisetin against Cyclophosphamide-induced Genotoxicity in Bone Marrow and Spleen Cells of Male Rats, International Journal of Drug Delivery Technology. 2023,13(2):468-473.
Shujuan Zhou, Lan Sun, Shanhu Qian, Yongyong Ma, Ruye Ma, Yuqing Dong, Yifen Shi, Songfu Jiang, Haige Ye, Zhijian Shen, Shenghui Zhang, Jianping Shen, Kang Yu and Siqian Wang, Iron overload adversely effects bone marrow haematogenesis via SIRT‑SOD2‑mROS in a process ameliorated by curcumin, Cellular & Molecular Biology Letters, 2021, 26:2.
M I Thabrewl, N Samarawickrema, L G Chandrasena and S Jayasekera, Effect of oral supplementation with vitamin E on the oxido-reductive status of red blood cells in normal mice and mice subject to oxidative stress by chronic administration of Adriamycin, Ann Clin Biochem 1999, 36: 216-220.
Safa A. Yahya, Nada N. Al-Shawi. Hepatoprotective effect of Nobiletin against 5-fluorouracil induce hepatotoxicity. Current Research in Pharmacology and Drug Discovery,2024, 100199.
Bauer, JD; Ackermann, PG, and Toro, G. Clinical Lab Methods. The C.V. Mosby Company, Saint Louis, 1995: 813-817.
Bancroft, JD and Marilyn, G (2008). Theory and practice of histological techniques. 1st Edn., London, Elsevier Limited. PP: 168-173.
Suad, K. A., Al-Shamire, J. S. H., & Dhyaa, A. A. Histological and biochemical evaluation of supplementing broiler diet with β-hydroxy-methyl butyrate calcium (β-HMB-Ca). Iranian journal of veterinary research, 2018, 19(1), 27.
Beutler E, Felitti V, Ho NJ, Gelbart T. Relationship of body iron stores to levels of serum ferritin, serum iron, unsaturated iron binding capacity, and transferrin saturation in patients with iron storage disease. Acta Haematol 2002,107:145 9.
Fung EB, Harmatz P, Milet M, Ballas SK, De Castro L, Hagar W, et al. Morbidity and mortality in chronically transfused subjects with thalassemia and sickle cell disease: A report from the multi center study of iron overload. Am J Hematol 2007, 82:255 65.
Vekeman, F. et al. Adherence to iron chelation therapy and associated healthcare resource utilization and costs in Medicaid patients with sickle cell disease and thalassemia. J. Med. Econ. 2016, 19: 292–303.
Baidaa I. Mohammed, Nada N. Al-Shawi, Ali F. Hasan, Protective Effects of Aqueous Cranberry Fruit Extract against Genotoxicity Induced by Cisplatin in Mice Bone Marrow Cells, International Journal of Drug Delivery Technology. 2023:13(1):401-406.
Knovich MA, Storey JA, Coffman LG, Torti SV, Torti FM. Ferritin for the clinician. Blood Rev 2009, 23:95 104.
Ethan Baratz, Olga Protchenko, Shyamalagauri Jadhav, Deliang Zhang, Pierre-Christian Violet, Samantha Grounds, Minoo Shakoury-Elizeh, Mark Levine, Caroline C. Philpott, Vitamin E Induces Liver Iron Depletion and Alters Iron Regulation in Mice, The Journal of Nutrition, 2023: 153(7).
George Papanikolaou, Michalis Tzilianos, John I. Christakis, Dionisios Bogdanos, Konstantina Tsimirika, Julie MacFarlane, Y. Paul Goldberg, Nikos Sakellaropoulos, Tomas Ganz, and Elizabeta Nemeth, Hepcidin in iron overload disorders 2005, 105: 4103-4105.
Nibras H. Jasem and Ali F. Hassan, Evaluation the Incidence of Genotoxic Effects of Artificial Food Favoring Additives in Bone Marrow Cells and Spleen Cells in Mice, Iraqi Journal of Pharmaceutical Sciences, 2020 :29(1).
W. Bradley Rittasea, Jeannie M. Muirb, John E. Slavena, Roxane M. Boutena, Michelle A. Bylickyc, W. Louis Wilkinsd, and Regina M. Daya, Deposition of Iron in the Bone Marrow of a Murine Model of Hematopoietic Acute Radiation Syndrome, Experimental Hematology, 2020, 84:54−66.
Ali Faris Hassan, Ruaa Fadhil Abbas, Nada Naji Al-Shawi, Intesar Tarik Numan, Evaluation of the Genoprotective and Cytoprotective Activity of Vitamin K-7 Against Doxorubicin in Bone Marrow Cells and Spleen Cells of Rats, Medico-Legal Update, 2020: 20, 4.
Fischer C, Volani C, Komlódi T, Seifert M, Demetz E. Valente de Souza L, et al. Dietary iron overload and Hfe-/- related hemochromatosis alter hepatic mitochondrial function. Antioxidants, 2021, 10:1818.
Tomasello G, Mazzola M, Leone A, Sinagra E, Zummo G, Farina F, et al. Nutrition, oxidative stress and intestinal dysbiosis: Influence of diet on gut microbiota in inflammatory bowel diseases. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 2016, 160:461–6.
Yoko Yagishita, Dionysios V. Chartoumpekis, Thomas W. Kensler, and Nobunao Wakabayashi, NRF2 and the Moirai: Life and Death Decisions on Cell Fates, ANTIOXIDANTS & REDOX SIGNALING, 2023, 38: 7–9.
Hye Kyoung Sung, Mayoorey Murugathasan, Ali A. Abdul-Sater and Gary Sweeney, Autophagy deficiency exacerbates iron overload induced reactive oxygen species production and apoptotic cell death in skeletal muscle cells, 2023, 14:252.
Ryo YAMAUCH, Vitamin E: Mechanism of Its Antioxidant Activity. Food Sct Technol. Int. Tokyo, 1997, 3 (4): 301-309.
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