Phytochemical Screening and Potential Cytotoxic Activity of Iraqi Corchorus olitorius Leaves Ethyl acetate Extract against The Breast and The Esophageal Cancer Cell Lines

Authors

  • Farah Kais Abdul-Wahab Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq
  • Zahraa Muhsin Hammodi Department of Pharmaceutics, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
  • Nabaa M. Ibrahim Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
  • Thukaa Z. ABDUL-JALIL Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq.

DOI:

https://doi.org/10.31351/vol35iss1pp220-228

Keywords:

Keywords: Breast cancer; Iraqi Corchorus olitorius; and Phenolic compounds .

Abstract

The Corchorus olitorius leaves, a popular 'healthier' meal in Iraq , provided cytotoxic effects against different cell lines. The main purpose of the research work was to assess the phytochemical composition and the cytotoxicity of the Corchorus olitorius leaf ethyl acetate extract. The Iraqi Corchorus olitorius leaves ethyl acetate extract was made and fractionated using the hot continuous soxhlet device technique.Following this, a number of general and specialized tests were used to qualitatively screen for the different phytochemical constitutions found within the plant extract. then the human Iraqi breast (AMJ13) and the esophagus (SKGT-4) were subjected to various ethyl acetate extract concentrations (31.2, 62.5 , 125 , 250, 500 and 1000 μg/ml) in order to perform The MTT assay, is also known as 3-(4,5 dimthylthiazol-2-yl)-2,5-dipheyltetrazolium bromide and the viability of the cell was subsequently evaluated 72 hours after the treatment .The Iraqi Corchorus olitorius leaf ethyl acetate extract exhibited cytotoxic activity against the AMJ 13 and SKGT-4 cell lines. It produced a dose-dependent action. For the AMJ13 and SKGT-4 cell lines, the values of the inhibitory concentrations at half- maximum (IC 50) were 139.5 µg / ml and 100.1 µg / ml, respectively. So from the results of the study conclude that the Iraqi Corchorus olitorius leaves ethyl acetate extract exhibited cytotoxic activity and decreased the viability of Iraqi human breast and esophageal cancer cell line. So that further research regarding this topic is required.

How to Cite

1.
Abdul-Wahab FK, Zahraa Muhsin Hammodi, Nabaa M. Ibrahim, Thukaa Z. ABDUL-JALIL. Phytochemical Screening and Potential Cytotoxic Activity of Iraqi Corchorus olitorius Leaves Ethyl acetate Extract against The Breast and The Esophageal Cancer Cell Lines. Iraqi Journal of Pharmaceutical Sciences [Internet]. 2026 Mar. 28 [cited 2026 Apr. 1];35(1):220-8. Available from: https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/4265

Publication Dates

Received

2024-11-26

Revised

2024-12-14

Accepted

2025-07-12

Published Online First

2026-03-29

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2026-03-28

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